LOCUS       sequence-141068-         761 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  tRNA synthetase/tRNA pair for the in vivo incorporation of 
            p-azido-l-phenylalanine, into proteins in E coli in response to the 
            amber codon, TAG.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 761)
  AUTHORS   Amiram M, Haimovich AD, Fan C, Wang YS, Aerni HR, Ntai I, Moonan DW,
            Ma NJ, Rovner AJ, Hong SH, Kelleher NL, Goodman AL, Jewett MC, Soll 
            D, Rinehart J, Isaacs FJ
  TITLE     Evolution of translation machinery in recoded bacteria enables 
            multi-site incorporation of nonstandard amino acids.
  JOURNAL   Nat Biotechnol. 2015 Dec;33(12):1272-1279. doi: 10.1038/nbt.3372. 
            Epub 2015 Nov 16.
  PUBMED    26571098
REFERENCE   2  (bases 1 to 761)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nbt.3372"; 
            journalName: "Nat Biotechnol"; date: "2015-12"; volume: "33"; issue:
            "12"; pages: "1272-1279"
COMMENT     Sequence Label: pEvol-pAzFRS.1.t1
FEATURES             Location/Qualifiers
     source          1..761
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 caactctcta ctgtttctcc atacccgttt ttttgggcta acaggaggaa ttagatctat
       61 ggacgaattt gaaatgataa agagaaacac atctgaaatt atcagcgagg aagagttaag
      121 agaggtttta aaaaaagatg aaaaatctgc tctgataggt tttgaaccaa gtggtaaaat
      181 acatttaggg cattatctcc aaataaaaaa gatgattgat ttacaaaatg ctggatttga
      241 tataattata ttgttggctg atttacacgc ctatttaaac cagaaaggag agttggatga
      301 gattagaaaa ataggagatt ataacaaaaa agtttttgaa gcaatggggt taaaggcaaa
      361 atatgtttat ggaagtgaat tccagcttga taaggattat acactgaatg tctatagatt
      421 ggctttaaaa actaccttaa aaagagcaag aaggagtatg gaacttatag caagagagga
      481 tgaaaatcca aaggttgctg aagttatcta tccaataatg caggttaatg ttatgcatta
      541 tgatggcgtt gatgtttatg ttggagggat ggagcagaga aaaatacaca tgttagcaag
      601 ggagctttta ccaaaaaagg ttgtttgtat tcacaaccct gtcttaacgg gtttggatgg
      661 agaaggaaag atgagttctt caaaagggaa ttttatagct gttgatgact ctccagaaga
      721 gattagggct aagataaaga aagcatactg cccagctgga g
//
