LOCUS       sequence-141112-        1776 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  PhlF-P3 gate.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 1776)
  AUTHORS   Nielsen AA, Der BS, Shin J, Vaidyanathan P, Paralanov V, Strychalski
            EA, Ross D, Densmore D, Voigt CA
  TITLE     Genetic circuit design automation.
  JOURNAL   Science. 2016 Apr 1;352(6281):aac7341. doi: 10.1126/science.aac7341.
  PUBMED    27034378
REFERENCE   2  (bases 1 to 1776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science."; 
            date: "2016-04-1"; volume: "352(6281):aac7341. doi"; pages: " 
            10.1126/science.aac7341"
COMMENT     Sequence Label: pPhlF-P3
FEATURES             Location/Qualifiers
     source          1..1776
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        join(18..23,24..39,40..46)
                     /label=tac promoter
                     /note="strong E. coli promoter; hybrid between the trp and 
                     lac UV5 promoters"
     protein_bind    52..71
                     /label=lac operator (symmetric)
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG). The 
                     symmetric lac operator was optimized for tight binding of 
                     lac repressor."
     misc_RNA        897..946
                     /label=sTRSV HHRz
                     /note="hammerhead ribozyme from the tobacco ringspot virus 
                     satellite RNA (Khvorova et al., 2003)"
     CDS             join(996..998,999..1001,1002..1715)
                     /codon_start=1
                     /product="enhanced YFP"
                     /label=EYFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTFGYGLQCFARYPDHMKLHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSYQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(1041..1062)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(1302..1321)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1649..1670
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 aacgatcgtt ggctgtgttg acaattaatc atcggctcgt ataatgtgtg gaattgtgag
       61 cgctcacaat tagcggtcaa cgcatgtgct ttgcgttctg atgagacagt gatgtcgaaa
      121 ccgcctctac aaataatttt gtttaacttt acgagggcga tcctatggca cgtaccccga
      181 gccgtagcag cattggtagc ctgcgtagtc cgcataccca taaagcaatt ctgaccagca
      241 ccattgaaat cctgaaagaa tgtggttata gcggtctgag cattgaaagc gttgcacgtc
      301 gtgccggtgc aagcaaaccg accatttatc gttggtggac caataaagca gcactgattg
      361 ccgaagtgta tgaaaatgaa agcgaacagg tgcgtaaatt tccggatctg ggtagcttta
      421 aagccgatct ggattttctg ctgcgtaatc tgtggaaagt ttggcgtgaa accatttgtg
      481 gtgaagcatt tcgttgtgtt attgcagaag cacagctgga ccctgcaacc ctgacccagc
      541 tgaaagatca gtttatggaa cgtcgtcgtg agatgccgaa aaaactggtt gaaaatgcca
      601 ttagcaatgg tgaactgccg aaagatacca atcgtgaact gctgctggat atgatttttg
      661 gtttttgttg gtatcgcctg ctgaccgaac agctgaccgt tgaacaggat attgaagaat
      721 ttaccttcct gctgattaat ggtgtttgtc cgggtacaca gcgttaagga aacacagaaa
      781 aaagcccgca cctgacagtg cgggcttttt ttttcgacca aaggtactcg acgtacggtg
      841 gaatctgatt cgttaccaat tgacatgata cgaaacgtac cgtatcgtta aggtagctgt
      901 caccggatgt gctttccggt ctgatgagtc cgtgaggacg aaacagcctc tacaaataat
      961 tttgtttaat actagagaaa gaggggaaat actagatggt gagcaagggc gaggagctgt
     1021 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
     1081 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
     1141 gcaccacagg caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc
     1201 tgcaatgctt cgcccgctac cccgaccaca tgaagctgca cgacttcttc aagtccgcca
     1261 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga
     1321 cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca
     1381 tcgacttcaa ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc
     1441 acaacgtcta tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc
     1501 gccacaacat cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccaa
     1561 tcggcgacgg ccccgtgctg ctgcccgaca accactacct tagctaccag tccgccctga
     1621 gcaaagaccc caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg
     1681 ggatcactct cggcatggac gagctgtaca agtaactcgg taccaaattc cagaaaagag
     1741 gcctcccgaa aggggggcct tttttcgttt tggtcc
//
