LOCUS       sequence-141114-         410 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Encodes an sgRNA for spCas9 driven by hU6 promoter with a modified 
            scaffold (Chen et al. Cell 2013) and BbsI sites that allow insertion
            of a spacer sequence.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 410)
  AUTHORS   Nelles DA, Fang MY, O'Connell MR, Xu JL, Markmiller SJ, Doudna JA, 
            Yeo GW
  TITLE     Programmable RNA Tracking in Live Cells with CRISPR/Cas9.
  JOURNAL   Cell. 2016 Mar 16. pii: S0092-8674(16)30204-5. doi: 
            10.1016/j.cell.2016.02.054.
  PUBMED    26997482
REFERENCE   2  (bases 1 to 410)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2016 
            Mar 16. pii: S0092-8674(16)30204-5. doi: 
            10.1016/j.cell.2016.02.054."
COMMENT     Sequence Label: pBluescriptSKII+ U6-sgRNA(F+E) empty
FEATURES             Location/Qualifiers
     source          1..410
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     promoter        51..299
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     51..71
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     222..241
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
ORIGIN
        1 taaaggaacc aattcagtcg actggatccg gtaccaaggt cgggcaggaa gagggcctat
       61 ttcccatgat tccttcatat ttgcatatac gatacaaggc tgttagagag ataattagaa
      121 ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat
      181 ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg
      241 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg
      301 ggtcttcgag aagacctgtt taagagctat gctggaaaca gcatagcaag tttaaataag
      361 gctagtccgt tatcaacttg aaaaagtggc accgagtcgg tgcttttttt
//
