LOCUS       sequence-141127-         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  T7 promoter and RBS  - derived from pET3a (5' CTAT/3' CATA) fusion).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-T7_RBS
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     7..24
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(48..105)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     RBS             145..167
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    182..206
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(206..225)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(207..225)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    265..284
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that start with ""ATG"""
     terminator      287..330
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     primer_bind     complement(334..352)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(580..599)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 gagcgcagcg agtcagtgag cgaggaagcc tgcataacgc gaagtaatct tttcggtttt
       61 aaagaaaaag ggcagggtgg tgacaccttg cccttttttg ccggactgca gctaatgaga
      121 cggcatgcgg tctcttatgg tatatctcct tcttaaagtt aaacaaaatt atttctagag
      181 gggaattgtt atccgctcac aattccccta tagtgagtcg tattaatttc gcgggatagt
      241 gagacccata tgcgtctcta gattctagaa gcggccgcga attccagaaa tcatccttag
      301 cgaaagctaa ggattttttt tatctgaaat tctgcctcgt gatacgccta tttttatagg
      361 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cactcgagtt gatcgggcac
      421 gtaagaggtt ccaactttca ccataatgaa ataagatcac taccgggcgt attttttgag
      481 ttatcgagat tttcaggagc taaggaagct aaaatggaga aaaaaatcac tggatatacc
      541 accgttgata tatcccaatg gcatcgtaaa gaacattttg aggcatttca gtcagttgct
      601 caatgtacct ataaccagac cgttcagctg gatattacgg cctttttaaa gaccgtaaag
      661 aaaaataagc acaagtttta tccggccttt attcacattc ttgcccgcct gatgaatgct
      721 catccggaat ttcgtatggc aatgaaagac ggtgagctgg tgatatggga tagtgttcac
      781 ccttgttaca ccgttttcca tgagcaaact gaaacgtttt catcgctctg gagtgaatac
      841 cacgacgatt tccggcagtt tctacacata tattcgcaag atgtggcgtg ttacggtgaa
      901 a
//
