LOCUS       sequence-141153-         811 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  RBS_L3.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 811)
  AUTHORS   Moore SJ, Lai HE, Kelwick RJ, Chee SM, Bell DJ, Polizzi KM, Freemont
            PS
  TITLE     EcoFlex: A Multifunctional MoClo Kit for E. coli Synthetic Biology.
  JOURNAL   ACS Synth Biol. 2016 May 2.
  PUBMED    27096716
REFERENCE   2  (bases 1 to 811)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2016 May 2."
COMMENT     Sequence Label: pBP-TL10
FEATURES             Location/Qualifiers
     source          1..811
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     6..23
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(47..104)
                     /label=his operon terminator
                     /note="This putative transcriptin terminator from the E. 
                     coli his operon has a 2-bp deletion introduced during 
                     synthesis. Its efficiency has not been determined."
     terminator      complement(132..159)
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     terminator      complement(175..246)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     complement(302..322)
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 agcgcagcga gtcagtgagc gaggaagcct gcataacgcg aagtaatctt ttcggtttta
       61 aagaaaaagg gcagggtggt gacaccttgc ccttttttgc cggactgcag cgtctctggc
      121 aaacatataa acgcagaaag gcccacccga aggtgagcca gtgtgactct agtagagagc
      181 gttcaccgac aaacaacaga taaaacgaaa ggcccagtct ttcgactgag cctttcgttt
      241 tatttgatgc ctggtcgatt atttgtatag ttcatccatg ccatgtgtaa tcccagcagc
      301 tgttacaaac tcaagaagga ccatgtgatc tctcttttcg ttgggatctt tcgaaagggc
      361 agattgtgtg gacaggtaat ggttgtctgg taaaaggaca gggccatcgc caattggagt
      421 attttgttga taatggtctg ctagttgaac gcttccatct tcaatgttgt gtctaatttt
      481 gaagttaact ttgattccat tcttttgttt gtctgccatg atgtatacat tgtgtgagtt
      541 atagttgtat tccaatttgt gtccaagaat gtttccatct tctttaaaat caataccttt
      601 taactcgatt ctattaacaa gggtatcacc ttcaaacttg acttcagcac gtgtcttgta
      661 gttcccgtca tctttgaaaa atatagttct ttcctgtaca taaccttcgg gcatggcact
      721 cttgaaaaag tcatgctgtt tcatatgatc tgggtatctc gcaaagcatt gaacaccata
      781 accgaaagta gtgacaagtg ttggccatgg a
//
