LOCUS       sequence-141221-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bicistronic expression of Magneto2.0 and mCherry in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Wheeler MA, Smith CJ, Ottolini M, Barker BS, Purohit AM, Grippo RM, 
            Gaykema RP, Spano AJ, Beenhakker MP, Kucenas S, Patel MK, Deppmann 
            CD, Guler AD
  TITLE     Genetically targeted magnetic control of the nervous system.
  JOURNAL   Nat Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265.
  PUBMED    26950006
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Neurosci. 2016 Mar 7. doi: 10.1038/nn.4265."
COMMENT     Sequence Label: pcDNA3.0-Magneto2.0-p2A-mCherry
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(109..165)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             complement(271..294)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctcgatctcg aactcgtggc cgttcacgga gccctccatg tgcaccttga agcgcatgaa
       61 ctccttgatg atggccatgt tatcctcctc gcccttgctc acggatccgg gaccggggtt
      121 ttcttccacg tctcctgctt gctttaacag agagaagttc gtggcgccgc tgcctgatcc
      181 cacgttgatg tcgatctggt ccagggggat gtactcgccc tcgctggtga ttctgctctt
      241 gctagccact cgggaggagc ctccaccccc tttgtcatcg tcgtctttat agtcgctccc
      301 gccacccccg cgtgcgcttt cattatcact gtctcccagg gtgtgcttgt caaagagata
      361 ttccgccaag ccagattcgg gcgctcccat cttgcgcaag ttggtcacgt ggtcacccaa
      421 ttctttgatg gctttcacct gctcattcag gtaatgtgtc tcaatgaagt cacacaaatg
      481 ggggtcattt ttgtcagtgg ccagtttgtg cagttccagt agtgactgat tcacattttt
      541 ttccaaatgt aatgcacact ccattgcatt cagcccgctc tcccagtcat cacagtctgg
      601 tttcttgata tcctgaagga agattcggcc acctcgttgg ttctgcagct tcatcagttt
      661 ctcagcatgt tccctctcct catgagattg gtgaagaaag tatttggcaa agttcttcaa
      721 agccacatca tcgcggtcaa agtagtaaga catggacagg taaacgtagg aggcgtagag
      781 ctccaggttg atctggcggt tgatggcggc ctctgagtcc tggtggtagt tctggcgcac
//
