LOCUS       sequence-141275-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express the inhibitory scaffold gephyrin tagged to teal 
            fluorescent protein. Expression is Cre dependant. Plasmid can also 
            be used to make lentivirus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Villa KL, Berry KP, Subramanian J, Cha JW, Oh WC, Kwon HB, Kubota Y,
            So PT, Nedivi E
  TITLE     Inhibitory Synapses Are Repeatedly Assembled and Removed at 
            Persistent Sites In Vivo.
  JOURNAL   Neuron. 2016 Feb 3. pii: S0896-6273(16)00011-8. doi: 
            10.1016/j.neuron.2016.01.010.
  PUBMED    26853302
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Feb 3. pii: S0896-6273(16)00011-8. doi: 
            10.1016/j.neuron.2016.01.010."
COMMENT     Sequence Label: FU- dio Teal-gephyrin - W
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(80..113)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(164..197)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     regulatory      205..214
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             211..918
                     /codon_start=1
                     /product="monomeric teal (cyan) fluorescent protein (Ai et 
                     al., 2006)"
                     /label=mTFP1
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEETTMGVIKPDMKIKLKMEGNVNGHAFVIEGEGEGKPYDG
                     TNTINLEVKEGAPLPFSYDILTTAFAYGNRAFTKYPDDIPNYFKQSFPEGYSWERTMTF
                     EDKGIVKVKSDISMEEDSFIYEIHLKGENFPPNGPVMQKKTTGWDASTERMYVRDGVLK
                     GDVXHKLLLEGGGHHRVDFKTIYRAKKAVKLPDYHFVDHRIEILNHDKDYNKVTVYESA
                     VARNSTDGMDELYK"
ORIGIN
        1 catagttaag aataccagtc aatctttcac aaattttgta atccagaggt tgattatcga
       61 taagcttgat atcgaattca taacttcgta tagcatacat tatacgaagt tattctttgc
      121 accattctaa agaataacag tgataatttc tgggttaagg caaataactt cgtataggat
      181 actttatacg aagttatgct agccgccacc atggtgagca agggcgagga gaccacaatg
      241 ggcgtaatca agcccgacat gaagatcaag ctgaagatgg agggcaacgt gaatggccac
      301 gccttcgtga tcgagggcga gggcgagggc aagccctacg acggcaccaa caccatcaac
      361 ctggaggtga aggagggagc ccccctgccc ttctcctacg acattctgac caccgcgttc
      421 gcctacggca acagggcctt caccaagtac cccgacgaca tccccaacta cttcaagcag
      481 tccttccccg agggctactc ttgggagcgc accatgacct tcgaggacaa gggcatcgtg
      541 aaggtgaagt ccgacatctc catggaggag gactccttca tctacgagat acacctcaag
      601 ggcgagaact tcccccccaa cggccccgtg atgcagaaga agaccaccgg ctgggacgcc
      661 tccaccgaga ggatgtacgt gcgcgacggc gtgctgaagg gcgacgtnna gcacaagctg
      721 ctgctggagg gcggcggcca ccaccgcgtt gacttcaaga ccatctacag ggccaagaag
      781 gcggtgaagc tgcccgacta tcactttgtg gaccaccgca tcgagatcct gaaccacgac
      841 aaggactaca acaaggtgac cgtttacgag agcgccgtgg cccgcaactc caccgacggc
      901 atggacgagc tgtacaagtc cggccggact cagatctcga gctcaagctt cgaattcggc
      961 acgagggg
//
