LOCUS       sequence-141279-         819 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Lamp1 fused to RFP. Used for localization to
            the lysosome..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 819)
  AUTHORS   Sherer NM, Lehmann MJ, Jimenez-Soto LF, Ingmundson A, Horner SM, 
            Cicchetti G, Allen PG, Pypaert M, Cunningham JM, Mothes W
  TITLE     Visualization of retroviral replication in living cells reveals 
            budding into multivesicular bodies.
  JOURNAL   Traffic 2003 Nov;4(11):785-801.
  PUBMED    14617360
REFERENCE   2  (bases 1 to 819)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Traffic"; 
            date: "2003-11"; volume: "4(11)"; pages: "785-801"
COMMENT     Sequence Label: Lamp1-RFP
FEATURES             Location/Qualifiers
     source          1..819
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     4..22
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     regulatory      149..158
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 cccttggtca ccttcagctt ggcggtctgg gtgccctcgt aggggcggcc ctcgccctcg
       61 ccctcgatct cgaactcgtg gccgttcacg gagccctcca tgcgcacctt gaagcgcatg
      121 aactccttga tgacgtcctc ggaggaggcc atggtggcga ccggtggatc ccggatggtc
      181 tgatagcccg cgtgactcct cttcctgccg atgaggtagg cgatgaggac gatgaggacc
      241 agccctgcca gggccccgcc cacagcaatg gggatcagca tgttgttacc gtcctgtaca
      301 cactcttcca cagacccaaa cctgtcactt tctaccctga aagcctggac ttgcacgctg
      361 aagacattga gggcgagcgc cttgctgaca aagatgtgct cctcactgtt gcacttgtat
      421 gagttgccga cactggcctg aagagctttc agggaatagt tggaggtgct gaacgtgggc
      481 tctatggcat caggaagagt catgttcaac tgaactcctt gcaggaaaaa caggctagaa
      541 gtggcattca tcccaaactg cagctccagg actctgctct tgttccccac cttcagggtc
      601 accaactggg caccgcaagt cccactatat ttgtcacttg ggttgatgtt gaatgctctg
      661 gtcacagtcg tgttgtcctt cttcatgtag gtgatgttga gttgcagtgc catagaggcc
      721 agcaggcagg ttccattgtc accagtcaca ttgtacttgg acacactggg gtttgtgggc
      781 acgagtggtg gtgaggggct gggtggccca gtagttggg
//
