LOCUS       sequence-141349-         801 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  NowGFP gene for bacterial expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 801)
  AUTHORS   Sarkisyan KS, Goryashchenko AS, Lidsky PV, Gorbachev DA, Bozhanova 
            NG, Gorokhovatsky AY, Pereverzeva AR, Ryumina AP, Zherdeva VV, 
            Savitsky AP, Solntsev KM, Bommarius AS, Sharonov GV, Lindquist JR, 
            Drobizhev M, Hughes TE, Rebane A, Lukyanov KA, Mishin AS
  TITLE     Green fluorescent protein with anionic tryptophan-based chromophore 
            and long fluorescence lifetime.
  JOURNAL   Biophys J. 2015 Jul 21;109(2):380-9. doi: 10.1016/j.bpj.2015.06.018.
  PUBMED    26200874
REFERENCE   2  (bases 1 to 801)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.bpj.2015.06"; journalName: "Biophys J"; date: 
            "2015-07-21- 21"; volume: "109"; issue: "2"; pages: "380-9"
COMMENT     Sequence Label: NowGFP /pQE-30
FEATURES             Location/Qualifiers
     source          1..801
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(52..54,55..768)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEKLFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKMSLK
                     FICTTGKLPVPWPTLKTTLTWGMQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGVDFKEDGNILGHKLEYNAISGNANITADKQKNGIKA
                     YFTIRHDVEDGSVLLADHYQQNTPIGDGPVLLPDNHYLSTQSKQSKDPNEKRDHMVLLE
                     FVTAAGIPLGADELYK"
     primer_bind     complement(94..115)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(355..374)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     702..723
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ataataaatt aactaatgag aggatcgcat caccagcacc atcacggatc cgtgagcaag
       61 ggcgagaagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg cgacgtaaac
      121 ggccacaagt tcagcgtgtc cggcgagggc gagggcgatg ccacctacgg caagatgtcc
      181 ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct caaaaccacc
      241 ctgacctggg gcatgcagtg cttcgcccgc taccccgacc acatgaagca gcacgacttc
      301 ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatcttctt caaggacgac
      361 ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt gaaccgcatc
      421 gagctgaagg gcgtcgactt caaggaggac ggcaacatcc tggggcacaa gctggagtac
      481 aacgccatca gcggcaacgc caatatcacc gccgacaagc agaagaacgg catcaaggcc
      541 tacttcacga tccgccacga cgtcgaggac ggcagcgtgc tgctcgccga ccactaccag
      601 cagaacaccc ccatcggcga cggccccgtg ctgctgcccg acaaccacta cctgagcacc
      661 cagtccaagc agagcaaaga ccccaacgag aagcgcgatc acatggtcct gctggagttc
      721 gtgaccgccg ccgggatccc tctcggcgcg gacgagctgt acaagtaaaa gcttaattag
      781 ctgagcttgg actcctgttg a
//
