LOCUS       sequence-141407-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid expressing fusion construct of Gal4 DBD and VP16 AD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Budzynski MA, Puustinen MC, Joutsen J, Sistonen L
  TITLE     Uncoupling Stress-Inducible Phosphorylation of Heat Shock Factor 1 
            from Its Activation.
  JOURNAL   Mol Cell Biol. 2015 Jul;35(14):2530-40. doi: 10.1128/MCB.00816-14. 
            Epub 2015 May 11.
  PUBMED    25963659
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00816-14"; journalName: "Mol Cell Biol"; date: 
            "2015-07"; volume: "35"; issue: "14"; pages: "2530-40"
COMMENT     Sequence Label: Gal4-VP16
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             93..533
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
     CDS             555..788
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
ORIGIN
        1 aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaagaggc ttttttggag
       61 gagatctaag cttgaagcaa gcctcctgaa agatgaagct actgtcttct atcgaacaag
      121 catgcgatat ttgccgactt aaaaagctca agtgctccaa agaaaaaccg aagtgcgcca
      181 agtgtctgaa gaacaactgg gagtgtcgct actctcccaa aaccaaaagg tctccgctga
      241 ctagggcaca tctgacagaa gtggaatcaa ggctagaaag actggaacag ctatttctac
      301 tgatttttcc tcgagaagac cttgacatga ttttgaaaat ggattcttta caggatataa
      361 aagcattgtt aacaggatta tttgtacaag ataatgtgaa taaagatgcc gtcacagata
      421 gattggcttc agtggagact gatatgcctc taacattgag acagcataga ataagtgcga
      481 catcatcatc ggaagagagt agtaacaaag gtcaaagaca gttgactgta tcgccggaat
      541 tcccggggat ctgggccccc ccgaccgatg tcagcctggg ggacgagctc cacttagacg
      601 gcgaggacgt ggcgatggcg catgccgacg cgctagacga tttcgatctg gacatgttgg
      661 gggacgggga ttccccgggt ccgggattta ccccccacga ctccgccccc tacggcgctc
      721 tggatatggc cgacttcgag tttgagcaga tgtttaccga tgcccttgga attgacgagt
      781 acggtgggta gggggcgcga ccggacccgc atcccccgtc tgggttttcc cctcccgtcc
      841 ccggttcgta tccacaataa acacgagcac atacattaca aaaacctgcg gttgtcgtct
      901 gattatttgg tggatccggg g
//
