LOCUS       sequence-141408-         995 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  plasmid expressing fusion construct of Gal4 DBD and VP16 AD.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 995)
  AUTHORS   Budzynski MA, Puustinen MC, Joutsen J, Sistonen L
  TITLE     Uncoupling Stress-Inducible Phosphorylation of Heat Shock Factor 1 
            from Its Activation.
  JOURNAL   Mol Cell Biol. 2015 Jul;35(14):2530-40. doi: 10.1128/MCB.00816-14. 
            Epub 2015 May 11.
  PUBMED    25963659
REFERENCE   2  (bases 1 to 995)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1128/MCB.00816-14"; journalName: "Mol Cell Biol"; date: 
            "2015-07"; volume: "35"; issue: "14"; pages: "2530-40"
COMMENT     Sequence Label: Gal4-VP16
FEATURES             Location/Qualifiers
     source          1..995
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             23..256
                     /codon_start=1
                     /gene="UL48"
                     /product="transcriptional activation domain of herpes 
                     simplex virus protein VP16 (Triezenberg et al., 1988; 
                     Cousens et al., 1989)"
                     /label=VP16 AD
                     /translation="APPTDVSLGDELHLDGEDVAMAHADALDDFDLDMLGDGDSPGPGF
                     TPHDSAPYGALDMADFEFEQMFTDALGIDEYGG"
     primer_bind     complement(408..427)
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     polyA_signal    452..500
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     complement(958..977)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 gccggaattc ccggggatct gggccccccc gaccgatgtc agcctggggg acgagctcca
       61 cttagacggc gaggacgtgg cgatggcgca tgccgacgcg ctagacgatt tcgatctgga
      121 catgttgggg gacggggatt ccccgggtcc gggatttacc ccccacgact ccgcccccta
      181 cggcgctctg gatatggccg acttcgagtt tgagcagatg tttaccgatg cccttggaat
      241 tgacgagtac ggtgggtagg gggcgcgacc ggacccgcat cccccgtctg ggttttcccc
      301 tcccgtcccc ggttcgtatc cacaataaac acgagcacat acattacaaa aacctgcggt
      361 tgtcgtctga ttatttggtg gatccggggg agatggggga ggctaactga aacacggaag
      421 gagacaatac cggaaggaac ccgcgctatg acggcaataa aaagacagaa taaaacgcac
      481 gggtgttggg tcgtttgttc ataaacgcgg ggttcggtcc cagggctggc actctgtcga
      541 taccccaccg agaccccatt ggggccaata cgcccgcgtt tcttcctttt ccccacccca
      601 ccccccaagt tcgggtgaag gcccagggct cgcagccaac gtcggggcgg caggccctgc
      661 catagccact ggccccgtgg gttagggacg gggtccccca tggggaatgg tttatggttc
      721 gtgggggtta ttattttggg cgttgcgtgg ggtcaggtcc acgacccaag cttgtcgacg
      781 gtaccccggg gaattcgagc tctagataag taatgatcat aatcagccat atcacatctg
      841 tagaggtttt acttgcttta aaaaacctcc cacacctccc cctgaacctg aaacataaaa
      901 tgaatgcaat tgttgttgtt aacttgttta ttgcagctta taatggttac aaataaagca
      961 atagcatcac aaatttcaca aataaagcat ttttt
//
