LOCUS       sequence-141427-         932 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  KanR with FRT sites + pT7::T710::CFP::T7 terminator::T1 terminator +
            ColE.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 932)
  AUTHORS   Shin JE, Lin C, Lim HN
  TITLE     Horizontal transfer of DNA methylation patterns into bacterial 
            chromosomes.
  JOURNAL   Nucleic Acids Res. 2016 Apr 15. pii: gkw230.
  PUBMED    27084942
REFERENCE   2  (bases 1 to 932)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Apr 15. pii: gkw230."
COMMENT     Sequence Label: pHL 1701
FEATURES             Location/Qualifiers
     source          1..932
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(387..420)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     primer_bind     455..474
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        455..473
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             505..527
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             535..567
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     primer_bind     complement(622..643)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(883..902)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 atccatcttg ttcaatcatg cgaaacgatc ctcatcctgt ctcttgatca gatcttgatc
       61 ccctgcgcca tcagatcctt ggcggcaaga aagccatcca gtttactttg cagggcttcc
      121 caaccttacc agagggcgcc ccagctggca attccggttc gcttgctgtc cataaaaccg
      181 cccagtctag ctatcgccat gtaagcccac tgcaagctac ctgctttctc tttgcgcttg
      241 cgttttccct tgtccagata gcccagtagc tgacattcat ccggggtcag caccgtttct
      301 gcggactggc tttctacgtg ttccgcttcc tttagcagcc cttgcgccct gagtgcttgc
      361 ggcagcgtga gcttcaaaag cgctctgaag ttcctatact ttctagagaa taggaacttc
      421 gaactgcagg tcgacggatc cccggaatga cgtctaatac gactcactat agggagacca
      481 caacggtttc cctctagaaa taattttgtt taactttaag aaggagatat acatatggct
      541 agcatgactg gtggacagca aatgggtcgc ggatccatgg tgagcaaggg cgaggagctg
      601 ttcaccgggg tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaggttc
      661 agcgtgtccg gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc
      721 tgcaccaccg gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctggggc
      781 gtgcagtgct tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc
      841 atgcccgaag gctacgtcca ggagcgtacc atcttcttca aggacgacgg caactacaag
      901 acccgcgccg aggtgaagtt cgagggcgac ac
//
