LOCUS       sequence-141463-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Cre-dependent reporter plasmid for bipolar cells in the retina.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Wang S, Sengel C, Emerson MM, Cepko CL
  TITLE     A gene regulatory network controls the binary fate decision of rod 
            and bipolar cells in the vertebrate retina.
  JOURNAL   Dev Cell. 2014 Sep 8;30(5):513-27. doi: 
            10.1016/j.devcel.2014.07.018. Epub 2014 Aug 21.
  PUBMED    25155555
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2014.07.018"; journalName: "Dev Cell"; date: 
            "2014-09-8- 8"; volume: "30"; issue: "5"; pages: "513-27"
COMMENT     Sequence Label: Chx10BP-LCL-EGFP
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             22..732
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(167..185)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     435..454
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     complement(824..843)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
ORIGIN
        1 gcatacatta tacgaagtta tatggtgagc aagggcgagg aggataacat ggccatcatc
       61 aaggagttca tgcgcttcaa ggtgcacatg gagggctccg tgaacggcca cgagttcgag
      121 atcgagggcg agggcgaggg ccgcccctac gagggcaccc agaccgccaa gctgaaggtg
      181 accaagggtg gccccctgcc cttcgcctgg gacatcctgt cccctcagtt catgtacggc
      241 tccaaggcct acgtgaagca ccccgccgac atccccgact acttgaagct gtccttcccc
      301 gagggcttca agtgggagcg cgtgatgaac ttcgaggacg gcggcgtggt gaccgtgacc
      361 caggactcct ccctgcagga cggcgagttc atctacaagg tgaagctgcg cggcaccaac
      421 ttcccctccg acggccccgt aatgcagaag aagaccatgg gctgggaggc ctcctccgag
      481 cggatgtacc ccgaggacgg cgccctgaag ggcgagatca agcagaggct gaagctgaag
      541 gacggcggcc actacgacgc tgaggtcaag accacctaca aggccaagaa gcccgtgcag
      601 ctgcccggcg cctacaacgt caacatcaag ttggacatca cctcccacaa cgaggactac
      661 accatcgtgg aacagtacga acgcgccgag ggccgccact ccaccggcgg catggacgag
      721 ctgtacaagt aaacacgtgc ggccgcactc ctcaggtgca ggctgcctat cagaaggtgg
      781 tggctggtgt ggccaatgcc ctggctcaca aataccactg agatcttttt ccctctgcca
      841 aaaattatgg ggacatcatg aagccccttg agcatctgac ttctggctaa taaaggaaat
      901 ttattttcat tgcaatagtg tgttggaatt ttt
//
