LOCUS       sequence-141692-         763 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  E. Coli/Gram-positive Bacterial Shuttle Vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 763)
  AUTHORS   Dunny GM, Lee LN, LeBlanc DJ
  TITLE     Improved electroporation and cloning vector system for gram-positive
            bacteria.
  JOURNAL   Appl Environ Microbiol. 1991 Apr;57(4):1194-201.
  PUBMED    1905518
REFERENCE   2  (bases 1 to 763)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "1991-04"; volume: "57(4)"; pages: 
            "1194-201"
COMMENT     Sequence Label: pDL276
FEATURES             Location/Qualifiers
     source          1..763
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    11..67
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(68..85)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(68..84)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(77..99)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(388..405)
                     /label=pBAD Reverse
                     /note="For vectors with E. coli araBAD promoter, reverse 
                     primer"
     terminator      558..644
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      736..763
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
ORIGIN
        1 tgattacgcc aagcttgcat gcctgcaggt cgactctaga ggatccccgg gtaccgagct
       61 cgaattcact ggccgtcgtt ttacaacgtc gtgactggga aaaccctggc gttacccaac
      121 ttaatcgcct tgcagcacat ccccctttcg ccagctggcg taatagcgaa gaggcccgca
      181 ccgatcgccc ttcccaacag ttgcgcagcc tgaatggcga atgccgggaa ttattcacca
      241 ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt
      301 ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcggctg
      361 ttttggcgga tgagagaaga ttttcagcct gatacagatt aaatcagaac gcagaagcgg
      421 tctgataaaa cagaatttgc ctggcggcag tagcgcggtg gtcccacctg accccatgcc
      481 gaactcagaa gtgaaacgcc gtagcgccga tggtagtgtg gggtctcccc atgcgagagt
      541 agggaactgc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt
      601 ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgccg ggagcggatt
      661 tgaacgttgc gaagcaacgg cccggagggt ggcgggcagg acgcccgcca taaactgcca
      721 ggcatcaaat taagcagaag gccatcctga cggatggcct ttt
//
