LOCUS       sequence-141728-        1051 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein Quantitation Reporter (PQR) to quantify a protein of 
            interest in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1051)
  AUTHORS   Lo CA, Kays I, Emran F, Lin TJ, Cvetkovska V, Chen BE
  TITLE     Quantification of Protein Levels in Single Living Cells.
  JOURNAL   Cell Rep. 2015 Dec 22;13(11):2634-44. doi: 
            10.1016/j.celrep.2015.11.048. Epub 2015 Dec 10.
  PUBMED    26686644
REFERENCE   2  (bases 1 to 1051)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.11.048"; journalName: "Cell Rep"; date: 
            "2015-12-22- 22"; volume: "13"; issue: "11"; pages: "2634-44"
COMMENT     Sequence Label: pCAG-GFP-PQRv3-RFP
FEATURES             Location/Qualifiers
     source          1..1051
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             726..782
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 atggtgtcca aaggagaaga actgttcact ggcgtcgtgc ccattctggt ggagctcgac
       61 ggggatgtga acgggcataa atttagtgtg agaggggagg gcgagggcga tgccactaac
      121 gggaagctta ccctcaagtt catttgtacc accggcaagc ttcctgtccc ctggcctacc
      181 ctcgtcacca cactcacata cggcgtgcag tgcttttcca ggtacccaga ccacatgaag
      241 cggcacgact tctttaagtc cgccatgccc gagggatacg tgcaggagcg gaccatcagc
      301 tttaaggatg acgggacata caagacaaga gccgaggtga agttcgaggg ggacactctg
      361 gtgaaccgga ttgagctgaa ggggattgat tttaaggagg atgggaacat cctgggccac
      421 aagctggagt acaactttaa ctcccataac gtgtacatca ccgccgacaa gcagaagaac
      481 gggatcaaag ccaacttcaa gatccggcac aacgtcgaag atggcagcgt ccagctggcc
      541 gatcactacc agcaaaacac accaattggg gacggacccg tgctgttgcc cgacaaccac
      601 tacttgagca cacagagcgt cttgagcaaa gacccaaacg agaagaggga tcacatggtc
      661 ttgacggaga gggtgactgc cgctgggatt acacacggca tggacgagct cttaagggaa
      721 gcggagcgac gaattttagt ctactgaaac aagcgggaga cgtggaggaa aaccctggac
      781 ctatggtgtc taagggcgaa gagctgatta aggagaacat gcacatgaag ctgtacatgg
      841 agggcaccgt gaacaaccac cacttcaagt gcacatccga gggcgaaggc aagccctacg
      901 agggcaccca gaccatgaga atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg
      961 acatcctggc taccagcttc atgtacggca gcagaacctt catcaaccac acccagggca
     1021 tccccgattt ctttaagcag tccttccctg a
//
