LOCUS       sequence-141729-        1029 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Protein Quantitation Reporter (PQR) to quantify a protein of 
            interest in mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1029)
  AUTHORS   Lo CA, Kays I, Emran F, Lin TJ, Cvetkovska V, Chen BE
  TITLE     Quantification of Protein Levels in Single Living Cells.
  JOURNAL   Cell Rep. 2015 Dec 22;13(11):2634-44. doi: 
            10.1016/j.celrep.2015.11.048. Epub 2015 Dec 10.
  PUBMED    26686644
REFERENCE   2  (bases 1 to 1029)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2015.11.048"; journalName: "Cell Rep"; date: 
            "2015-12-22- 22"; volume: "13"; issue: "11"; pages: "2634-44"
COMMENT     Sequence Label: pCAG-GFP-PQRv3-RFP
FEATURES             Location/Qualifiers
     source          1..1029
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     85..104
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     CDS             complement(185..916)
                     /codon_start=1
                     /product="photostable variant of TagRFP (Shaner et al., 
                     2008)"
                     /label=TagRFP-T
                     /translation="MVSKGEELIKENMHMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTM
                     RIKVVEGGPLPFAFDILATSFMYGSRTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGG
                     VLTATQDTSLQDGCLIYNVKIRGVNFPSNGPVMQKKTLGWEANTEMLYPADGGLEGRTD
                     MALKLVGGGHLICNFKTTYRSKKPAKNLKMPGVYYVDHRLERIKEADKETYVEQHEVAV
                     ARYCDLPSKLGHKLNGMDELYK"
     CDS             complement(917..973)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
ORIGIN
        1 tccaacacac tattgcaatg aaaataaatt tcctttatta gccagaagcc agatgctcaa
       61 ggggcttcat gatgtcccca taatttttgg cagagggaaa aagatctcag tggtatttgt
      121 gagccagggc attggccaca ccagccacca ccttctgata ggcagcctgc acctgaggag
      181 tttacttgta cagctcgtcc atgccattaa gtttgtgccc cagtttgcta gggaggtcgc
      241 agtatctggc cacagccacc tcgtgctgct cgacgtaggt ctctttgtcg gcctccttga
      301 ttctttccag tctgtggtcc acatagtaga cgccgggcat cttgaggttc ttagcgggtt
      361 tcttggatct gtatgtggtc ttgaagttgc agatcaggtg gcccccgccc acgagcttca
      421 gggccatgtc ggttctgcct tccaggccgc cgtcagcggg gtacagcatc tcggtgttgg
      481 cctcccagcc gagtgttttc ttctgcatca cagggccgtt ggatgggaag ttcacccctc
      541 tgatcttgac gttgtagatg aggcagccgt cctggaggct ggtgtcctgg gtagcggtca
      601 gcacgccccc gtcttcgtat gtggtgactc tctcccatgt gaagccctca gggaaggact
      661 gcttaaagaa atcggggatg ccctgggtgt ggttgatgaa ggttctgctg ccgtacatga
      721 agctggtagc caggatgtcg aaggcgaagg ggagagggcc gccctcgacc accttgattc
      781 tcatggtctg ggtgccctcg tagggcttgc cttcgccctc ggatgtgcac ttgaagtggt
      841 ggttgttcac ggtgccctcc atgtacagct tcatgtgcat gttctcctta atcagctctt
      901 cgcccttaga caccataggt ccagggtttt cctccacgtc tcccgcttgt ttcagtagac
      961 taaaattcgt cgctccgctt cccttaagag ctcgtccatg ccgtgtgtaa tcccagcggc
     1021 agtcaccct
//
