LOCUS       sequence-141853-         715 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Intermediate cloning vector, with MCS2 containing inducible unstable
            peptide driven by pSTL1 (UbiY 2xNLSs SZ1)..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 715)
  AUTHORS   Aymoz D, Wosika V, Durandau E, Pelet S
  TITLE     Real-time quantification of protein expression at the single-cell 
            level via dynamic protein synthesis translocation reporters.
  JOURNAL   Nat Commun. 2016 Apr 21;7:11304. doi: 10.1038/ncomms11304.
  PUBMED    27098003
REFERENCE   2  (bases 1 to 715)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2016-04-21"; volume: "7:11304. doi"; pages: " 
            10.1038/ncomms11304"
COMMENT     Sequence Label: pDA125
FEATURES             Location/Qualifiers
     source          1..715
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(228..248)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(264..284)
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             complement(420..647)
                     /codon_start=1
                     /product="Saccharomyces cerevisiae ubiquitin"
                     /label=ubiquitin
                     /translation="MQIFVKTLTGKTITLEVESSDTIDNVKSKIQDKEGIPPDQQRLIF
                     AGKQLEDGRTLSDYNIQKESTLHLVLRLRGG"
     primer_bind     complement(673..689)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(673..689)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
ORIGIN
        1 ttcttacgca gattcgcgat ttctttctcc aggtacgcga tcaggtcttt tttgtgcagg
       61 ttctttttct tcagggtttc gttctcattt tccagagacg caacttcgtt ttcgagctgc
      121 gcaaccaggt tagatctttt aaattccatt gtaaaaggtg ctgcggcact tccagctttt
      181 ttatataagg aagtttgatt gctgccaccg tcgacactag atccccccac cttccgcttc
      241 ttcttgggtt cagcaccagc ccccaccttc cgcttcttct tgggttcgga tctcataagc
      301 tttccctgca ttaaagaatc ggccaaagcc ctgggggaag ctgtttgagt attaggtgct
      361 agggtcgttt ttctctttac caaactaact ggaagcagcc acgcacctga tttatggtaa
      421 ccacctctta gccttagcac aagatgtaag gtggactcct tctgaatgtt gtaatcagac
      481 agcgttctac cgtcttctag ctgcttaccg gcaaagatca atctttgttg atctggaggg
      541 ataccttcct tgtcttgaat tttcgactta acgttgtcga tggtatcgga agattcaact
      601 tccaatgtta tggttttacc ggtcaaagtc ttgacgaaaa tctgcatgat atcgaattcc
      661 tgcagcccgg gggatccact agttctagag gtctaaaact ttctatgttc tattt
//
