LOCUS       sequence-141918-       10470 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  mCherry^SEC^3xFlag vector with ccdB markers for cloning homology 
            arms.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10470)
  AUTHORS   Ramalho JJ, Sepers JJ, Nicolle O, Schmidt R, Cravo J, Michaux G, 
            Boxem M
  TITLE     C-terminal phosphorylation modulates ERM-1 localization and dynamics
            to control cortical actin organization and support lumen formation 
            during Caenorhabditiselegans development.
  JOURNAL   Development. 2020 Jul 22;147(14):dev188011. doi: 10.1242/dev.188011.
  PUBMED    32586975
REFERENCE   2  (bases 1 to 10470)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Development."; date: "2020-07-22"; volume: "147(14):dev188011. 
            doi"; pages: " 10.1242/dev.188011"
COMMENT     Sequence Label: pJJR83
FEATURES             Location/Qualifiers
     source          1..10470
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     364..386
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     378..395
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     379..395
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     CDS             661..966
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     CDS             1896..1916
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     protein_bind    complement(1934..1967)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             1977..2006
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     terminator      complement(3823..4146)
                     /gene="tbb-2"
                     /label=tbb-2 terminator
                     /note="transcription terminator for the C. elegans tubulin 
                     beta-2 gene"
     protein_bind    6064..6088
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             6091..7116
                     /codon_start=1
                     /product="hygromycin B phosphotransferase"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     CDS             6091..7116
                     /codon_start=1
                     /gene="aph(4)-Ia"
                     /product="aminoglycoside phosphotransferase from E. coli"
                     /label=HygR
                     /note="confers resistance to hygromycin"
                     /translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
                     YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRAQGVTLQDLP
                     ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
                     TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
                     GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
                     NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
                     E"
     protein_bind    complement(7117..7141)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(7494..7527)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             7546..7569
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7573..7596
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             7600..7623
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(7671..7976)
                     /codon_start=1
                     /gene="ccdB"
                     /product="CcdB, a bacterial toxin that poisons DNA gyrase"
                     /label=ccdB
                     /note="Plasmids containing the ccdB gene cannot be 
                     propagated in standard E. coli strains."
                     /translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
                     VPRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
     primer_bind     complement(8249..8265)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8249..8265)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8262..8284)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8273..8289
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8297..8303,8304..8321,8322..8327))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8342..8363
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8480..8497)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8651..9239)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8731..8750)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9410..10201,10202..10270))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10033..10052
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10271..10375)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10443..10461
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatgcg gtgtgaaata ccgcacagat gcgtaaggag aaaataccgc atcaggcgcc
      241 attcgccatt caggctgcgc aactgttggg aagggcgatc ggtgcgggcc tcttcgctat
      301 tacgccagct ggcgaaaggg ggatgtgctg caaggcgatt aagttgggta acgccagggt
      361 tttcccagtc acgacgttgt aaaacgacgg ccagtcgccg gcactagtta cccgaagtat
      421 gtcaaaaaga ggtatgctat gaagcagcgt attacagtga cagttgacag cgacagctat
      481 cagttgctca aggcatatat gatgtcaata tctccggttc ggtaagcaca accatgcaga
      541 atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga aaatcaggaa gggatggctg
      601 aggtcgcccg gtttattgaa atgaacggct cttttgccga cgagaacagg ggctggtgaa
      661 atgcagttta aggtttacac ctataaaaga gagagccgtt atcgtctgtt tgtggatgta
      721 cagagtgata ttattgacac gcccgggcga cggatggtga tccccctggc cagtgcacgt
      781 ctgctgtcag ataaagtccc ccgtgaactt tacccggtgg tgcatatcgg ggatgaaagc
      841 tggcgcatga tgaccaccga tatggccagt gtgccggtct ccgttatcgg ggaagaagtg
      901 gctgatctca gccaccgcga aaatgacatc aaaaacgcca ttaacctgat gttctgggga
      961 atataaatgt caggctccct tatacacagc cagtctgcag gtcgacctag ggagcatcgg
     1021 gagcctcagg agcatcgatg tccaagggag aggaggacaa catggccatc atcaaggagt
     1081 tcatgcgttt caaggtccac atggagggat cagtcaacgg acacgagttc gagatcgagg
     1141 gagagggaga gggacgtcca tacgagggaa cccaaaccgc caagctcaag gtaagtttaa
     1201 acatatatat actaactaac cctgattatt taaattttca ggtcaccaag ggaggaccac
     1261 tcccattcgc ctgggacatc ctctccccac aattcatgta cggatcaaag gcctacgtca
     1321 agcacccagc cgacatccca gactacctca agctctcctt cccagaggga ttcaagtggg
     1381 agcgtgtcat gaacttcgag gacggaggag tcgtcaccgt cacccaagac tcctccctcc
     1441 aagacggaga gttcatctac aaggtaagtt taaacagttc ggtactaact aaccatacat
     1501 atttaaattt tcaggtcaag ctccgtggaa ccaacttccc atccgacgga ccagtcatgc
     1561 aaaagaagac catgggatgg gaggcctcct ccgagcgtat gtacccagag gacggagccc
     1621 tcaagggaga gatcaagcaa cgtctcaagc tcaaggacgg aggacactac gacgccgagg
     1681 tcaagaccac ctacaaggcc aagaagccag tccaactccc aggtaagttt aaacatgatt
     1741 ttactaacta actaatctga tttaaatttt caggagccta caacgtcaac atcaagctcg
     1801 acatcacctc ccacaacgag gactacacca tcgtcgagca atacgagcgt gccgagggac
     1861 gtcactccac cggaggaatg gacgagctct acaaggagaa tctgtacttt caatccggaa
     1921 aggtaagttt aaaataactt cgtatagcat acattatacg aagttatttt cagggagagc
     1981 aaaagcttat ctccgaggag gacctctaag gatgatcgac gccaacgtcg ttgaattttc
     2041 aaattttaaa tactgaatat ttgttttttt tcctattatt tatttattct ctttgtgttt
     2101 tttttcttgc tttctaaaaa attaattcaa tccaaatcta aacatttttt tttctctttc
     2161 cgtctcccaa ttcgtattcc gctcctctca tctgaacaca atgtgcaagt ttatttatct
     2221 tctcgctttc atttcattag gacgtggggg gaattggtgg aagggggaaa cacacaaaag
     2281 gatgatggaa atgaaataag gacacacaat atgcaacaac attcaattca gaaatatgga
     2341 ggaaggttta aaagaaaaca taaaaatata tagaggagga aggaaactag ctgccctcga
     2401 ttgtctatct tccattctca ccaagtttca acaactttat tcagtcattc ttctctggtt
     2461 catttcccaa ccaatcttcc cgaataaagc ccatctgtcc tttcggccac attcctcacc
     2521 tacatccgta ctccttatct cccgttctta tccagtcttt tatcgtctct gcgtctcgca
     2581 cactgtctcg tcggtaatgt aaaaaaaaaa caaaaaaaac ttgaaaactt gcggaaagat
     2641 ttcctaataa aaggagacga atccggtagg aaatcgacaa gtctctgctt ctcgaagact
     2701 aggtgagtta catttctttg gccgaattat tttgaaatcc taaatttgat aatttcagag
     2761 atgtctgtaa aacttgcgtg ttatgtgacg gcttcggtca ctgtcgccac tcttatggta
     2821 tgcttcatga ccatgtcaac catctactcg gaggttgatg gattcagaga gaagcttgat
     2881 accgagatga atgtgttcag agtgagttaa attatatttt gaattttaaa taatttttaa
     2941 ttttctagca atctaccaat ggattgtgga aggacatagt tgtcatcgga agatctagca
     3001 agcgtgtccg ttgtcaatat gaagagacca acgctacccc aactccacat gctgatggat
     3061 ccccatctgc tccaccaggt caaccaccag cagttccacc agtcttcaac cagccaaaga
     3121 ctccaaatgg agccaatgga aatggaccaa cctgcaactg caatgctgat aacaagtgcc
     3181 cagctggacc atccggacca aagggagttc caggagttcc aggactcgac ggagttccag
     3241 gacttgacgg tgttccagga gttggagctg atgatatcgc tccacaacgc gagtctgtcg
     3301 gatgcttcac ttgcccacaa ggaccagttg gaccaccagg agctcttgga agaccaggac
     3361 cacgtggact tccaggacca agaggacaaa atggaaaccc aggaagagat ggacaaccag
     3421 gacatccagg agagcaagga tcatccggtc aaatcggaaa gatcggagag ccaggaccac
     3481 caggagagaa gggacgcgac gccgagcatc caatcggaag accaggacca aagggaccaa
     3541 gaggagatca aggaccaaca ggaccagctg gacagaacgg tcttcacgga ccaccaggag
     3601 agccaggaac cgttggacca gaaggaccat ctggaaagca aggacgtcaa ggaccagacg
     3661 gaacccaggg agagactgga ccagacggaa gaccaggaaa ggatgccgag tactgccagt
     3721 gcccagacaa gtctccacca tcagaggctg tcaacgccaa ccgtggatac agaaatatct
     3781 aaattgttgg tgttttctaa taaaatattt gagattcagt gagacttttt tcttggcggc
     3841 acaataaagt ttcctctttt tgttgggtca ctcaacgaac acaatacaaa tatttgagcg
     3901 ctcgcatttt tctcaacctt tcgggtttaa agcacttcaa aatggaattg aagagagatt
     3961 gtgaaagtac atcagacaga atcacatttt gatagcattc acttcactca gatgcaaact
     4021 tttcagtgac tataaaaagt cataaaataa taacattaaa aaagcaaata aattagcgag
     4081 agaatttttt gacaaaaaga aagaagagtg atagagaaga agggaatgct tgaaaggatt
     4141 ttgcatttat cttaatcacc gtcttcgaga agacgaacca tggcgccagt ttcagaatcc
     4201 aagtttctaa tatagttcat cacaatgttg acgttggtcc aacctccggc ttgcataatc
     4261 tctgggatgg aaactccagc gcgagccatg tcacgggcag ctccgacacg ggcggaatgt
     4321 ccagaccaag ccaagtaacg ttgtccggaa tcatccttgg ctccgtagat gagacggtgg
     4381 gtagcttcga agattcctga aaatttattt tcagcttatt cttagctaat atgttgttta
     4441 aacttacctt caagagctcg agtcgagagt tgtgatgttg cactcggggc agcaactccg
     4501 ttcttgcgaa cacgacagaa caggtagttg tttggatcat cggcgactcc ggaaaccgaa
     4561 atccagcgct caacgagctt ggtaactcca agggagagcg ctttttcgac tccggcagtc
     4621 gagaccagcg tttttgtacg tccgatgtgg atgagcattc ttcctccgtc tgtgcgacta
     4681 atatccttga cgcgaatgcg ggcaatttcg gcgatacgga gaagtgtgtt gtaagcaatt
     4741 ccgaggaagg cgaggtttcg gatgtcctgg caacgatcgg aattttccat aagagaacgg
     4801 acctgatcga agtcggttct gaaaatttcg tcaagtctta ggattgaata cattaatatt
     4861 taaacttacc tttcgaaggc gagggcttgc ttggctctct ctccagcatc aacattttcc
     4921 ttgcggatac gtctcattac gagagatacg gcattagaat ctgatgggcg tggaagtcca
     4981 gatcggcgat ggagcatatt gagctgtccg agatgttgtt ggatcgtctt caccgccaat
     5041 ccacgagctt ggaggtagag caagtagtca cgaacgtctt ctggctcggc tgggaaccac
     5101 ttgcggttgt taagcttgca ccaggcagcc caagaacggc acactgagag aagcatcttc
     5161 caggtgtgct cggagaaggc ttgtctgtca cggaacatat ccatgaggtt cttgcgaact
     5221 tcatcacttg tcgcgtcaac tggaagtgct ggaagatttt gatgaacagt gagaagattt
     5281 gacatatttt cgaagttttt tagatgcact agaacaaagc gtgttggctt cctctgagcc
     5341 cgctttcctt atatacccgc attctgcagc cttacagaat gttctagaag gtcctagatg
     5401 cattcgtttg aaaatactcc cggtgggtgc aaagagacgc agacggaaaa tgtatctggg
     5461 tctctttatt gtgtacacta cttttccatg taccgaatgt gagtcgccct ccttttgcaa
     5521 caagcagctc gaatgttcta gaaaaaggtg gaaaatagta taaataccgt tgaaaataaa
     5581 taccgaacaa catttgctct aattgtgaaa ttagaaatct tcaaactata atcatgtcac
     5641 tttaccacta tttccgtcca gctcaacgtt ccgtttttgg tgatcatttt tgctttcgtc
     5701 gtaaatctac acacgcgtct cttccgtgcg agagtccaag ccagcagcca aattcgttga
     5761 ctgagtattc aacgtttata cgttgtcggc aacgagaaat aggaaaatgc atcgggaaat
     5821 gttctttttt cgattttttc caaggttttg acaaatttta ccacgaattt tgctatgttt
     5881 tcaattaaaa aatatgttat tcaactgttt ctatgaggaa aataaggctt tgcatgtaat
     5941 tttcttattc agcataattt ttaattaatt tgaattttct gtcctaacgt ttattttgtt
     6001 ttcttggtta tgactgatct gaaattaatt tttgaatttt aaggtaatat gtcaggcggt
     6061 gccgcaagtt tgtacaaaaa agcaggctcc atgaaaaagc ctgaactcac cgcgacgtct
     6121 gtcgagaagt ttctgatcga aaagttcgac agcgtctccg acctgatgca gctctcggag
     6181 ggcgaagaat ctcgtgcttt cagcttcgat gtaggagggc gtggatatgt cctgcgggta
     6241 aatagctgcg ccgatggttt ctacaaagat cgttatgttt atcggcactt tgcatcggcc
     6301 gcgctcccga ttccggaagt gcttgacatt ggggaattca gcgagagcct gacctattgc
     6361 atctcccgcc gtgcacaggg tgtcacgttg caagacctgc ctgaaaccga actgcccgct
     6421 gttctgcagc cggtcgcgga ggccatggat gcgatcgctg cggccgatct tagccagacg
     6481 agcgggttcg gcccattcgg accgcaagga atcggtcaat acactacatg gcgtgatttc
     6541 atatgcgcga ttgctgatcc ccatgtgtat cactggcaaa ctgtgatgga cgacaccgtc
     6601 agtgcgtccg tcgcgcaggc tctcgatgag ctgatgcttt gggccgagga ctgccccgaa
     6661 gtccggcacc tcgtgcacgc ggatttcggc tccaacaatg tcctgacgga caatggccgc
     6721 ataacagcgg tcattgactg gagcgaggcg atgttcgggg attcccaata cgaggtcgcc
     6781 aacatcttct tctggaggcc gtggttggct tgtatggagc agcagacgcg ctacttcgag
     6841 cggaggcatc cggagcttgc aggatcgccg cggctccggg cgtatatgct ccgcattggt
     6901 cttgaccaac tctatcagag cttggttgac ggcaatttcg atgatgcagc ttgggcgcag
     6961 ggtcgatgcg acgcaatcgt ccgatccgga gccgggactg tcgggcgtac acaaatcgcc
     7021 cgcagaagcg cggccgtctg gaccgatggc tgtgtagaag tactcgccga tagtggaaac
     7081 cgacgcccca gcactcgtcc gagggcaaag gaatagaccc agctttcttg tacaaagtgg
     7141 gtccaattac tcttcaacat ccctacatgc tctttctccc tgtgctccca ccccctattt
     7201 ttgttattat caaaaaactt ctcttaattt ctttgttttt tagcttcttt taagtcacct
     7261 ctaacaatga aattgtgtag attcaaaaat agaattaatt cgtaataaaa agtcgaaaaa
     7321 aattgtgctc cctcccccca ttaataataa ttctatccca aaatctacac aatgttctgt
     7381 gtacacttct tatgtttttt acttctgata aatttttttg aaacatcata gaaaaaaccg
     7441 cacacaaaat accttatcat atgttacgtt tcagtttatg accgcaattt ttaataactt
     7501 cgtatagcat acattatacg aagttatttt cagggagccg gatctgatta taaagacgat
     7561 gacgataagc gtgactacaa ggacgacgac gacaagcgtg attacaagga tgacgatgac
     7621 aagagactag ttcgacctgc agactggctg tgtataaggg agcctgacat ttatattccc
     7681 cagaacatca ggttaatggc gtttttgatg tcattttcgc ggtggctgag atcagccact
     7741 tcttccccga taacggagac cggcacactg gccatatcgg tggtcatcat gcgccagctt
     7801 tcatccccga tatgcaccac cgggtaaagt tcacggggga ctttatctga cagcagacgt
     7861 gcactggcca gggggatcac catccgtcgc ccgggcgtgt caataatatc actctgtaca
     7921 tccacaaaca gacgataacg gctctctctt ttataggtgt aaaccttaaa ctgcatttca
     7981 ccagcccctg ttctcgtcgg caaaagagcc gttcatttca ataaaccggg cgacctcagc
     8041 catcccttcc tgattttccg ctttccagcg ttcggcacgc agacgacggg cttcattctg
     8101 catggttgtg cttaccgaac cggagatatt gacatcatat atgccttgag caactgatag
     8161 ctgtcgctgt caactgtcac tgtaatacgc tgcttcatag catacctctt tttgacatac
     8221 ttcgggttcc taggaaatcg ataacatggt catagctgtt tcctgtgtga aattgttatc
     8281 cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     8341 aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     8401 acctgtcgtg ccagctgcat taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta
     8461 ttgggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     8521 gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     8581 caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     8641 tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     8701 gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     8761 ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     8821 cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     8881 tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     8941 tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     9001 cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     9061 agtggtggcc taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga
     9121 agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     9181 gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     9241 aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     9301 ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     9361 gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     9421 taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     9481 tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     9541 tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     9601 gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     9661 gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     9721 ttgctacagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     9781 cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     9841 tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     9901 cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     9961 agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
    10021 cgtcaatacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
    10081 aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
    10141 aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
    10201 gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
    10261 gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
    10321 tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
    10381 ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
    10441 aaaataggcg tatcacgagg ccctttcgtc
//
