LOCUS       sequence-142000-         668 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of mutant human codon-optimized dCas9-DNMT3A fusion 
            (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning 
            backbone for sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 668)
  AUTHORS   Vojta A, Dobrinic P, Tadic V, Bockor L, Korac P, Julg B, Klasic M, 
            Zoldos V
  TITLE     Repurposing the CRISPR-Cas9 system for targeted DNA methylation.
  JOURNAL   Nucleic Acids Res. 2016 Mar 11. pii: gkw159.
  PUBMED    26969735
REFERENCE   2  (bases 1 to 668)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Mar 11. pii: gkw159."
COMMENT     Sequence Label: pdCas9-DNMT3A-PuroR (ANV)_v2
FEATURES             Location/Qualifiers
     source          1..668
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             19..42
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             49..69
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 tacaaggatc atgatattga ttacaaagac gatgacgata agatggcccc aaagaagaag
       61 cggaaggtcg gtatccacgg agtcccagca gccgacaaga agtacagcat cggcctggcc
      121 atcggcacca actctgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag
      181 aaattcaagg tgctgggcaa caccgaccgg cacagcatca agaagaacct gatcggagcc
      241 ctgctgttcg acagcggcga aacagccgag gccacccggc tgaagagaac cgccagaaga
      301 agatacacca gacggaagaa ccggatctgc tatctgcaag agatcttcag caacgagatg
      361 gccaaggtgg acgacagctt cttccacaga ctggaagagt ccttcctggt ggaagaggat
      421 aagaagcacg agcggcaccc catcttcggc aacatcgtgg acgaggtggc ctaccacgag
      481 aagtacccca ccatctacca cctgagaaag aaactggtgg acagcaccga caaggccgac
      541 ctgcggctga tctatctggc cctggcccac atgatcaagt tccggggcca cttcctgatc
      601 gagggcgacc tgaaccccga caacagcgac gtggacaagc tgttcatcca gctggtgcag
      661 acctacaa
//
