LOCUS       sequence-142002-         815 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of mutant human codon-optimized dCas9-DNMT3A fusion 
            (inactive catalytic domain of DNMT3A) with T2A-PuroR (v2); cloning 
            backbone for sgRNA.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 815)
  AUTHORS   Vojta A, Dobrinic P, Tadic V, Bockor L, Korac P, Julg B, Klasic M, 
            Zoldos V
  TITLE     Repurposing the CRISPR-Cas9 system for targeted DNA methylation.
  JOURNAL   Nucleic Acids Res. 2016 Mar 11. pii: gkw159.
  PUBMED    26969735
REFERENCE   2  (bases 1 to 815)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nucleic 
            Acids Res. 2016 Mar 11. pii: gkw159."
COMMENT     Sequence Label: pdCas9-DNMT3A-PuroR (ANV)_v2
FEATURES             Location/Qualifiers
     source          1..815
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 gttagcagac ttcctctgcc ctctccactg ccgaattcgg gccggccgga cacacacgca
       61 aaatactcct tcagcggagc gaagaggtgg cggatgactg gcacgctcca tgaccggccc
      121 agcagtctct gcctcgccaa gcggctcatg ttggagacgt cagtatagtg gactgggaaa
      181 ccaaataccc tttccatttc agtgcaccat aagatgtcct ctttctcatt catgaacaca
      241 ggaaaatgct ggtctttgcc ctgctttatg gagtttgacc tcgtagtaat ggtcctcact
      301 ttgctgaact tggctatcct gccatgctcc agacactcct gcagctccag cttatcattc
      361 acagtggatg ccaacggcct gttcataccg ggaaggttac cccagaagta gcgggccctg
      421 tgtgcagctg acacttcttt ggcatcaatc atcacagggt tggactcgag aaatcgcgag
      481 atgtccctct tgtcactaac gcccatggcc accacattcg caaagagcca gaagaagggg
      541 cgatcatctc cctccttggg ccgcgcatca tgcaggaggc ggtagaactc aaagaagagc
      601 cggccagtgc cctcgtagag gcccttgcga gcagggttga cgatggagag gtcattgcag
      661 ggactgcccc caatcaccag atcgaatggg ccccactcct ggatatgctt ctgtgtgacg
      721 ctgcggacgt ccccgacgta catgatcttc ccctggtgcc gcaccatgcc caccgtgatg
      781 gagtcctcac acacctccga ggcaatgtag cggtc
//
