LOCUS       sequence-142068-        1893 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Retroviral expression plasmid encoding a human BRD9 bromodomain-swap
            mutant containing the first bromodomain of BRD4 with a C terminal 3x
            FLAG-tag.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1893)
  AUTHORS   Hohmann AF, Martin LJ, Minder JL, Roe JS, Shi J, Steurer S, Bader G,
            McConnell D, Pearson M, Gerstberger T, Gottschamel T, Thompson D, 
            Suzuki Y, Koegl M, Vakoc CR
  TITLE     Sensitivity and engineered resistance of myeloid leukemia cells to 
            BRD9 inhibition.
  JOURNAL   Nat Chem Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115.
  PUBMED    27376689
REFERENCE   2  (bases 1 to 1893)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Chem 
            Biol. 2016 Jul 4. doi: 10.1038/nchembio.2115."
COMMENT     Sequence Label: MSCV-Cterm_3xFL_BRD9_BET-PGK-Puro-IRES-GFP
FEATURES             Location/Qualifiers
     source          1..1893
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             1323..1334
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     CDS             1825..1890
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 atgggcaaga agcacaagaa gcacaaggcc gagtggcgct cgtcctacga ggattatgcc
       61 gacaagcccc tggagaagcc tctaaagcta gtcctgaagg tcggaggaag tgaagtgact
      121 gaactctcag gatccggcca cgactccagt tactatgatg acaggtcaga ccatgagcga
      181 gagaggcaca aagaaaagaa aaagaagaag aagaagaagt ccgagaagga gaagcatctg
      241 gacgatgagg aaagaaggaa gcgaaaggaa gagaagaagc ggaagcgaga gagggagcac
      301 tgtgacacgg agggagaggc tgacgacttt gatcctggga agaaggtgga ggtggagccg
      361 cccccagatc ggccagtccg agcgtgccgg acacagccag ccgaaaatga gagcacaagg
      421 cagaccaacc aactgcaata cctgctcaga gtggtgctca agacactatg gaaacaccag
      481 tttgcatggc ctttccagca gcctgtggat gccgtcaagc tgaacctccc tgattactat
      541 aagatcatta aaacgcctat ggatatggga acaataaaga agcgcttgga aaacaactat
      601 tactggaatg ctcaggaatg tatccaggac ttcaacacta tgtttacaaa ttgttacatc
      661 tacaacaagc ctggagatga catagtctta atggcagaag ctctggaaaa gctcttcttg
      721 caaaaaataa atgagctaag caaacaggca gctcttttgg gcaatgaaga tacagctgtt
      781 gaggaacctg tccctgaagt tgtaccagta caagtagaaa ctgccaagaa atccaaaaag
      841 ccgagtagag aagttatcag ctgcatgttt gagcctgaag ggaatgcctg cagcttgacg
      901 gacagtaccg cagaggagca cgtgctggcg ctggtggagc acgcagctga cgaagctcgg
      961 gacaggatca accggttcct cccaggcggc aagatgggct atctgaagag gaacggggac
     1021 gggagcctgc tctacagcgt ggtcaacacg gccgagccgg acgctgatga ggaggagacc
     1081 cacccggtgg acttgagctc gctctccagt aagctactcc caggcttcac cacgctgggc
     1141 ttcaaagacg agagaagaaa caaagtcacc tttctctcca gtgccactac tgcgctttcg
     1201 atgcagaata attcagtatt tggcgacttg aagtcggacg agatggagct gctctactca
     1261 gcctacggag atgagacagg cgtgcagtgt gcgctgagcc tgcaggagtt tgtgaaggat
     1321 gctgggagct acagcaagaa agtggtggac gacctcctgg accagatcac aggcggagac
     1381 cactctagga cgctcttcca gctgaagcag agaagaaatg ttcccatgaa gcctccagat
     1441 gaagccaagg ttggggacac cctaggagac agcagcagct ctgttctgga gttcatgtcg
     1501 atgaagtcct atcccgacgt ttctgtggat atctccatgc tcagctctct ggggaaggtg
     1561 aagaaggagc tggaccctga cgacagccat ttgaacttgg atgagacgac gaagctcctg
     1621 caggacctgc acgaagcaca ggcggagcgc ggcggctctc ggccgtcgtc caacctcagc
     1681 tccctgtcca acgcctccga gagggaccag caccacctgg gaagcccttc tcgcctgagt
     1741 gtcggggagc agccagacgt cacccacgac ccctatgagt ttcttcagtc tccagagcct
     1801 gcggcctctg ccaagaccgg aggcgattac aaagaccatg acggtgatta taaagatcat
     1861 gatatcgatt acaaggatga cgatgacaag tga
//
