LOCUS       sequence-142091-         849 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression vector containing flag tagged WT MFF isoform 
            5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 849)
  AUTHORS   Toyama EQ, Herzig S, Courchet J, Lewis TL Jr, Loson OC, Hellberg K, 
            Young NP, Chen H, Polleux F, Chan DC, Shaw RJ
  TITLE     Metabolism. AMP-activated protein kinase mediates mitochondrial 
            fission in response to energy stress.
  JOURNAL   Science. 2016 Jan 15;351(6270):275-81. doi: 10.1126/science.aab4138.
  PUBMED    26816379
REFERENCE   2  (bases 1 to 849)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-01-15- 15"; volume: "351"; 
            issue: "6270"; pages: "275-81"
COMMENT     Sequence Label: pLenti.PGK.blast-Flag-MFF-WT
FEATURES             Location/Qualifiers
     source          1..849
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    83..107
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             116..139
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 tgaccgaatc accgacctct ctccccaggg ggatccaccg gttcgtcgac tagtccagtg
       61 tggtggaatt ctgcagatat caacaagttt gtacaaaaaa gcaggcttca ccatggatta
      121 caaggacgac gatgacaagg gcggatctat ggcagaaatt agtcgaattc agtacgaaat
      181 ggaatatact gaaggcatta gtcagcgaat gagggtccca gaaaagttaa aagtagcacc
      241 gccaaacgct gacctggaac aaggattcca agaaggagtt ccaaatgcta gtgtgataat
      301 gcaagttccg gagaggattg ttgtagcagg aaataatgaa gatgtttcat tttcaagacc
      361 agcagatctt gaccttattc agtcaactcc ctttaaaccc ctggcactga aaacaccacc
      421 tcgtgtactt acgctgagtg aaagaccact agattttctg gatttagaaa gacctcctac
      481 aacccctcaa aatgaagaaa tccgagcagt tggcagacta aaaagagagc ggtctatgag
      541 tgaaaatgct gttcgccaaa atggacagct ggtcagaaat gattctctac ctgtgttgcg
      601 tggtgggtct gctgccgcca cttctaatcc tcatcatgac aacgtcaggt atggcatttc
      661 aaatatagat acaaccattg aaggaacgtc agatgacctg actgttgtag atgcagcttc
      721 actaagacga cagataatca aactaaatag acgtctacaa cttctggaag aggagaacaa
      781 agaacgtgct aaaagagaaa tggtcatgta ttcaattact gtagctttct ggctgcttaa
      841 tagctggct
//
