LOCUS       sequence-142092-         930 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Lentiviral expression vector containing flag tagged WT MFF isoform 
            5.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 930)
  AUTHORS   Toyama EQ, Herzig S, Courchet J, Lewis TL Jr, Loson OC, Hellberg K, 
            Young NP, Chen H, Polleux F, Chan DC, Shaw RJ
  TITLE     Metabolism. AMP-activated protein kinase mediates mitochondrial 
            fission in response to energy stress.
  JOURNAL   Science. 2016 Jan 15;351(6270):275-81. doi: 10.1126/science.aab4138.
  PUBMED    26816379
REFERENCE   2  (bases 1 to 930)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1126/science"; 
            journalName: "Science"; date: "2016-01-15- 15"; volume: "351"; 
            issue: "6270"; pages: "275-81"
COMMENT     Sequence Label: pLenti.PGK.blast-Flag-MFF-WT
FEATURES             Location/Qualifiers
     source          1..930
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    60..84
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             complement(812..835)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     protein_bind    complement(844..868)
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 tttccaattt tgtaatccag aggttgattg tcgagcggcc gccactgtgc tggatatcaa
       61 ccactttgta caagaaagct gggtcctagc ggcgaaacca gagccagcta ttaagcagcc
      121 agaaagctac agtaattgaa tacatgacca tttctctttt agcacgttct ttgttctcct
      181 cttccagaag ttgtagacgt ctatttagtt tgattatctg tcgtcttagt gaagctgcat
      241 ctacaacagt caggtcatct gacgttcctt caatggttgt atctatattt gaaatgccat
      301 acctgacgtt gtcatgatga ggattagaag tggcggcagc agacccacca cgcaacacag
      361 gtagagaatc atttctgacc agctgtccat tttggcgaac agcattttca ctcatagacc
      421 gctctctttt tagtctgcca actgctcgga tttcttcatt ttgaggggtt gtaggaggtc
      481 tttctaaatc cagaaaatct agtggtcttt cactcagcgt aagtacacga ggtggtgttt
      541 tcagtgccag gggtttaaag ggagttgact gaataaggtc aagatctgct ggtcttgaaa
      601 atgaaacatc ttcattattt cctgctacaa caatcctctc cggaacttgc attatcacac
      661 tagcatttgg aactccttct tggaatcctt gttccaggtc agcgtttggc ggtgctactt
      721 ttaacttttc tgggaccctc attcgctgac taatgccttc agtatattcc atttcgtact
      781 gaattcgact aatttctgcc atagatccgc ccttgtcatc gtcgtccttg taatccatgg
      841 tgaagcctgc ttttttgtac aaacttgttg atatctgcag aattccacca cactggacta
      901 gtcgacgaac cggtggatcc ccctggggga
//
