LOCUS       sequence-142126-         827 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 827)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 827)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: AKAP13
FEATURES             Location/Qualifiers
     source          1..827
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             12..34
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             45..62
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             87..107
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tctagaataa ttttgtttaa ctttaagaag gagatataca tatgcaccat catcatcatc
       61 attcttctgg tgtagatctg ggtaccgaga acctgtactt ccaatccatg gaagcagagt
      121 cttggagtcg gataatagac agcaagtttc taaaacagca aaagaaagat gtggtcaaac
      181 ggcaagaagt aatatatgag ttgatgcaga cagagtttca tcatgtccgc actctcaaga
      241 tcatgagtgg tgtgtacagc caggggatga tggcggatct gctttttgag cagcagatgg
      301 tagaaaagct gttcccctgt ttggatgagc tgatcagtat ccatagccaa ttcttccaga
      361 ggattctgga gcggaagaag gagtctctgg tggataaaag tgaaaagaac tttctcatca
      421 agaggatagg ggatgtgctt gtaaatcagt tttcaggtga gaatgcagaa cgtttaaaga
      481 agacatatgg caagttttgt gggcaacata accagtctgt aaactacttc aaagaccttt
      541 atgccaagga taagcgtttt caagcctttg taaagaagaa gatgagcagt tcagttgtta
      601 gaaggcttgg aattccagag tgcatattgc ttgtaactca gcggattacc aagtacccag
      661 ttttattcca aagaatattg cagtgtacca aagacaatga agtggagcag gaagatctag
      721 cacagtcctt gagcctggtg aaggatgtga ttggagctgt agacagcaaa gtggcaagtt
      781 atgaaaagaa agtgcgtctc aatgagattt atacaaagtg acagtaa
//
