LOCUS       sequence-142130-         858 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 858)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 858)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: FAM83A
FEATURES             Location/Qualifiers
     source          1..858
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(33..50)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(668..688)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(713..730)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             741..763
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     protein_bind    778..802
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     complement(802..821)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(803..821)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 ctctttcggg ctttgttagc agccggatct cagtggtggt ggtggtggtg ctcgagtgcg
       61 gccgcaagct tgtcgacgga gctcgaattc ggatccgtat ccacctttac tgtcacagcc
      121 gcggggactt caggcccatc acaggcttgg aggaggcgta gaggtggcgg aactcctcgt
      181 caaacagctc caccgcctgg cctgtgaact tggagaggat gttccggtgc acgtgtccgc
      241 agagccaggt gaagctgtaa gatccagaca ggacaaatct ccagtccgag atgatgaact
      301 tctcccggat ttggccagcg aatttcctgc ctgacttggc acagtatatc tctccttcca
      361 cactccggat ggaaatgttc ttgaggtgac tgtcagagat ctggactttg tcacacatct
      421 cctggaagag cttcacacct ccctggtcca ggagcacaca aacgaacacc ccacgcttgt
      481 tggctgcctc tagaatgtca cagaagatct ccacatccgt gaacacatcc atcaggatga
      541 ccaggacctg gctagtccgg gtgatgcagc ggcggacgag gtctctgatg ttgttgtgct
      601 tgacggtctg gaagtacaca gtggcgctgg atttttcctt caggtagggc ttctcagctg
      661 aggccatgga ttggaagtac aggttctcgg tacccagatc tacaccagaa gaatgatgat
      721 gatgatggtg catatgtata tctccttctt aaagttaaac aaaattattt ctagagggga
      781 attgttatcc gctcacaatt cccctatagt gagtcgtatt aaatttcgcg ggatcgagat
      841 ctcgatcctc tacgccgg
//
