LOCUS       sequence-142177-        1059 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express the inhibitory scaffold gephyrin tagged to teal 
            fluorescent protein. Expression is Cre dependant. Plasmid can also 
            be used to make lentivirus..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1059)
  AUTHORS   Villa KL, Berry KP, Subramanian J, Cha JW, Oh WC, Kwon HB, Kubota Y,
            So PT, Nedivi E
  TITLE     Inhibitory Synapses Are Repeatedly Assembled and Removed at 
            Persistent Sites In Vivo.
  JOURNAL   Neuron. 2016 Feb 3. pii: S0896-6273(16)00011-8. doi: 
            10.1016/j.neuron.2016.01.010.
  PUBMED    26853302
REFERENCE   2  (bases 1 to 1059)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2016 Feb 3. pii: S0896-6273(16)00011-8. doi: 
            10.1016/j.neuron.2016.01.010."
COMMENT     Sequence Label: FU- dio Teal-gephyrin - W
FEATURES             Location/Qualifiers
     source          1..1059
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(178..211)
                     /label=lox2272
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (AAGTATCC) (Shaw et al., 2021). lox2272 sites are 
                     compatible with each other, but incompatible with loxP or 
                     loxN sites (Lee and Saito, 1988)."
     protein_bind    complement(262..295)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
ORIGIN
        1 gtgtgttttg tgaagttttt taggcacctt ttgaaatgta atcatttggg tcaatatgta
       61 attttcagtg ttagactagt aaattgtccg ctaaattctg gccgtttttg gcttttttgt
      121 tagacgaagc ttgggctgca ggtcgactct agaggatcct ctagagtcga ctccggaata
      181 acttcgtata ggatacttta tacgaagtta tgcagaatgg tagctggatt gtagctgcta
      241 ttagcaatat gaaacctctt aataacttcg tatagcatac attatacgaa gttatggcgc
      301 gcccgtacgt catagccgtc cgatgaccat gacgtccacc acctcgcctt tgtgaagttc
      361 cacatactgc tctgtctttg gaggtagcat cagcaatcca ttggcactgc gcatgctcat
      421 cagacggctg ctcatctgat tacctgtact ctgggcccag ggcagtggtt cttggtgatg
      481 ccaagtcagt atacaccggt ggtattctgg gcgagggtcc agctttacgt cacatgataa
      541 cctagctttg atgatggttg gccgaggatc caagataccc tgcatttttc tcagtgcggg
      601 tacaacaaag aggttgcagg tgaccacagc tgatacagga ttccctggta gtgcaaaaat
      661 aatttttctt acaccatcaa tatccaaagt tgcaaatgtt gttggcaagc ctggcttcat
      721 aaaaaccctg ccaaaatgga tctgagcatg aagatcgatg tccagcacct gcttgagata
      781 gtccttttcc cccatggata cacctcctga cgtgatgatg acatcagcac ggctgatacc
      841 ctcattcaag gcattgagta agtcatctgg gttgtctcct acaattccca agttgattgt
      901 tgggtaacca tgttcctgaa ttgttgctag aagtgttgat cgattgctgt cccgaatctt
      961 tcctggtaag aggtcatctt caggatttag tagctcattc cctgttgaca taacggcaac
     1021 cactggaaac ttattaactt caacttctgt gacacctac
//
