LOCUS       sequence-142331-         957 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FlAsH-BRET reporter to monitor conformational changes in Arrestin3. 
            Insertion of FlAsH motif (CCPGCC) following amino acid residue 40 of
            Arrestin3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 957)
  AUTHORS   Lee MH, Appleton KM, Strungs EG, Kwon JY, Morinelli TA, Peterson YK,
            Laporte SA, Luttrell LM
  TITLE     The conformational signature of beta-arrestin2 predicts its 
            trafficking and signalling functions.
  JOURNAL   Nature. 2016 Mar 31;531(7596):665-8. doi: 10.1038/nature17154. Epub 
            2016 Mar 23.
  PUBMED    27007854
REFERENCE   2  (bases 1 to 957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17154"; journalName: "Nature"; date: "2016-03-31- 
            31"; volume: "531"; issue: "7596"; pages: "665-8"
COMMENT     Sequence Label: Rlucrarr3-Flash1
FEATURES             Location/Qualifiers
     source          1..957
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    555..676
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(592..611)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     646..665
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(820..841)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 caccataacc ccgctgctca gtgacaaccg agagaagcgt ggccttgccc ttgatgggca
       61 actcaagcac gaagacacca acctggcttc cagcaccatt gtgaaggagg gagccaacaa
      121 ggaggtgctg ggaatcctag tatcctacag ggtcaaggtg aagctggtgg tgtctcgagg
      181 cggggatgtc tccgtggagc tacctttcgt cctaatgcac cccaagcccc acgaccacat
      241 cacccttccc cgaccccagt cagccccccg ggaaatagac atccctgtgg ataccaacct
      301 cattgaattc gataccaact atgccacaga cgacgacatc gtgtttgagg actttgcgag
      361 gcttcggctg aaggggatga aggatgacga ctgtgatgac cagttctgct agtctagagg
      421 gcccgggatc caccggatct agataactga tcataatcag ccataccaca tttgtagagg
      481 ttttacttgc tttaaaaaac ctcccacacc tccccctgaa cctgaaacat aaaatgaatg
      541 caattgttgt tgttaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
      601 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
      661 tcatcaatgt atcttaacgc gtaaattgta agcgttaata ttttgttaaa attcgcgtta
      721 aatttttgtt aaatcagctc attttttaac caataggccg aaatcggcaa aatcccttat
      781 aaatcaaaag aatagaccga gatagggttg agtgttgttc cagtttggaa caagagtcca
      841 ctattaaaga acgtggactc caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc
      901 ccactacgtg aaccatcacc ctaatcaagt ttttggggtc gaggtgccgt aaagcat
//
