LOCUS       sequence-142467-         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses mouse Eps8 fused to GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  AUTHORS   Frittoli E, Matteoli G, Palamidessi A, Mazzini E, Maddaluno L, 
            Disanza A, Yang C, Svitkina T, Rescigno M, Scita G
  TITLE     The signaling adaptor Eps8 is an essential actin capping protein for
            dendritic cell migration.
  JOURNAL   Immunity. 2011 Sep 23;35(3):388-99. doi: 
            10.1016/j.immuni.2011.07.007. Epub 2011 Aug 11.
  PUBMED    21835647
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.immuni.2011.07.007"; journalName: "Immunity"; date: 
            "2011-09-23- 23"; volume: "35"; issue: "3"; pages: "388-99"
COMMENT     Sequence Label: LZRSpBMN-­Z‐GFP‐Eps8
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(128..150)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    139..155
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(163..169,170..187,188..193))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    208..229
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(346..363)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(597..616)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atagggcgaa ttcgagctcg ggtacccggg gatcctctag agctcgacct gcaggcatgt
       61 caaggcgttg agtattctag tagtgtcacc taaatagctt ggcgctaatc atggtcatag
      121 ctgatttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
      181 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
      241 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
      301 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
      361 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcagagg cggtaatacg
      421 gttatccaca gaatcagggg ataacgcatg aaagaacatg tgagcaaaag gccagcaaaa
      481 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
      541 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
      601 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
      661 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
      721 ctgtaggtat ctca
//
