LOCUS       sequence-142468-         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Gene targeting vector for the mouse Rosa26 locus, including a loxP 
            flanked stop cassette and BFP, for gateway cloning.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Chu VT, Weber T, Graf R, Sommermann T, Petsch K, Sack U, Volchkov P,
            Rajewsky K, Kuhn R
  TITLE     Efficient generation of Rosa26 knock-in mice using CRISPR/Cas9 in 
            C57BL/6 zygotes.
  JOURNAL   BMC Biotechnol. 2016 Jan 16;16(1):4. doi: 10.1186/s12896-016-0234-4.
  PUBMED    26772810
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC 
            Biotechnol."; date: "2016-01-16"; volume: "16(1):4. doi"; pages: " 
            10.1186/s12896-016-0234-4"
COMMENT     Sequence Label: pR26 iBFP Dest
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(48..81)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    90..297
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 cgccgggatc actctcggca tggacgagct gtacaagtaa ccccggggaa gttcctatac
       61 tttctagaga ataggaactt cgctagagac tgtgccttct agttgccagc catctgttgt
      121 ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg tcctttccta
      181 ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc tggggggtgg
      241 ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg ctggggatgc
      301 ggtctagcgc ggccgccgcg ccgatatcga attcgctaga agatgggcgg gagtcttctg
      361 ggcaggctta aaggctaacc tggtgtgtgg gcgttgtcct gcaggggaat tgaacaggtg
      421 taaaattgga gggacaagac ttcccacaga ttttcggttt tgtcgggaag ttttttaata
      481 ggggcaaata aggaaaatgg gaggataggt agtcatctgg ggttttatgc agcaaaacta
      541 caggttatta ttgcttgtga tccgcctcgg agtattttcc atcgaggtag attaaagaca
      601 tgctcacccg agttttatac tctcctgctt gagatcctta ctacagtatg aaattacagt
      661 gtcgcgagtt agactatgta agcagaattt taatcatttt taaagagccc agtacttcat
      721 atccatttct cccgctcctt ctgcagcctt atcaaa
//
