LOCUS       sequence-142497-         734 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 734)
  TITLE     SGC 2016 plasmids
REFERENCE   2  (bases 1 to 734)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: KCTD13A
FEATURES             Location/Qualifiers
     source          1..734
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             3..25
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             36..53
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             78..98
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             521..538
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     primer_bind     complement(591..609)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      605..652
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 attttgttta actttaagaa ggagatatac atatgcacca tcatcatcat cattcttctg
       61 gtgtagatct gggtaccgag aacctgtact tccaatccat gggccccgcc gcctacggtc
      121 tcaagccgct gaccccgaac agcaaatacg tgaagctgaa cgtgggcggc tcgttgcact
      181 acaccacgct gcgcaccctc acgggacagg acaccatgct caaagccatg ttcagcggcc
      241 gcgtggaggt gctgaccgat gccggaggtt gggtgctgat tgaccggagc ggccgtcact
      301 ttggtacaat cctcaattac ctgcgggatg ggtctgtgcc actgccggag agtacgagag
      361 aactggggga gctgctgggc gaagcacgct actacctggt gcagggcctg attgaggact
      421 gccagctggc gctgcagcaa aaaagggaga cgctgtgaca gtaaaggtgg atacggatcc
      481 gaattcgagc tccgtcgaca agcttgcggc cgcactcgag caccaccacc accaccactg
      541 agatccggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
      601 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
      661 aggaactata tccggattgg cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg
      721 ggtgtggtgg ttac
//
