LOCUS       sequence-142562-         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses His tagged LanCL2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Zeng M, van der Donk WA, Chen J
  TITLE     Lanthionine synthetase C-like protein 2 (LanCL2) is a novel 
            regulator of Akt.
  JOURNAL   Mol Biol Cell. 2014 Dec 1;25(24):3954-61. doi: 
            10.1091/mbc.E14-01-0004. Epub 2014 Oct 1.
  PUBMED    25273559
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1091/mbc.E14-01-0004"; journalName: "Mol Biol Cell"; date: 
            "2014-12-1- 1"; volume: "25"; issue: "24"; pages: "3954-61"
COMMENT     Sequence Label: pET28b-LanCL2 (6xHis-LanCL2)
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             43..60
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             70..87
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
ORIGIN
        1 attttgttta ctttaagaag gagatatacc atgggcagca gccatcatca tcatcatcac
       61 agcagcggcc tggtgccgcg cggcagccat atgggcgaga ccatgtcaaa gaggctgaag
      121 ctccacctgg gaggggaggc agaaatggag gaacgggcgt tcgtcaaccc cttcccggac
      181 tacgaggccg ccgccggggc gctgctcgcc tccggagcgg ccgaagagac aggctgtgtt
      241 cgtcccccgg ccaccccgga tgagcccggc ctcccttttc atcaggacgg gaagatcatt
      301 cataatttca taagacggat ccagaccaaa attaaagatc ttctgcagca aatggaagaa
      361 gggctgaaga cagctgatcc ccatgactgc tctgcttata ctggctggac aggcatagcc
      421 cttttgtacc tgcagttgta ccgggtcaca tgtgaccaaa cctacctgct ccgatccctg
      481 gattacgtaa aaagaacact tcggaatctg aatggccgca gggtcacctt cctctgtggg
      541 gatgctggcc ccctggctgt tggagctgtg atttatcaca aactcagaag tgactgtgag
      601 tcccaggaat gtgtcacaaa acttttgcag ctccagagat cggttgtctg ccaagaatca
      661 gaccttcctg atgagctgct ttatggacgg gcaggttatc tgtatgcctt actgtacctg
      721 aacacagaga taggtccagg caccgtgtgt gagtcagcta
//
