LOCUS       sequence-142586-        9696 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Constitutive ETV4 ORF, blasticidin selection.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9696)
  AUTHORS   Wang B, Krall EB, Aguirre AJ, Kim M, Widlund HR, Doshi MB, Sicinska 
            E, Sulahian R, Goodale A, Cowley GS, Piccioni F, Doench JG, Root DE,
            Hahn WC
  TITLE     ATXN1L, CIC, and ETS Transcription Factors Modulate Sensitivity to 
            MAPK Pathway Inhibition.
  JOURNAL   Cell Rep. 2017 Feb 7;18(6):1543-1557. doi: 
            10.1016/j.celrep.2017.01.031.
  PUBMED    28178529
REFERENCE   2  (bases 1 to 9696)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.celrep.2017.01"; journalName: "Cell Rep"; date: 
            "2017-02-7- 7"; volume: "18"; issue: "6"; pages: "1543-1557"
COMMENT     Sequence Label: pLX_TRC311 ETV4-L
FEATURES             Location/Qualifiers
     source          1..9696
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        98..1276
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          329..1267
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     1224..1244
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     protein_bind    1296..1320
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     protein_bind    complement(2793..2817)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     CDS             2825..2866
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     misc_feature    2917..3505
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2970..2990)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3388..3399)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             3577..3810
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    3888..4009
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3925..3944)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3979..3998
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4049..4184
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4204..4223)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4205..4223)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4233..4250)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4233..4249)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4242..4264)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      4391..4846
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4478..4497)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4688..4709
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        4872..4976
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(4977..5045,5046..5837)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5195..5214)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6008..6596
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6497..6516
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6750..6767
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    6884..6905
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6920..6925,6926..6943,6944..6950)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6958..6974
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6963..6985
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     6982..6998
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6982..6998
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7017..7037
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7019..7037
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7065..7291
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7292..7472
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    7519..7644
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8137..8370
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             8555..8599
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     promoter        8830..9040
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     promoter        8852..9048
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8899..9034
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             9070..9465
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
     misc_feature    9539..9656
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 ggctcgaggc ctgcaggtgc aaagatggat aaagttttaa acagagagga atctttgcag
       61 ctaatggacc ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc
      121 agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg
      181 tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct
      241 ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt
      301 tcgcaacggg tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg
      361 cctctttacg ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt
      421 gattcttgat cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta
      481 aggagcccct tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt
      541 gcgaatctgg tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta
      601 aaatttttga tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg
      661 ccaagatctg cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc
      721 gtcccagcgc acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg
      781 ggggtagtct caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc
      841 cccgccctgg gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc
      901 gcttcccggc cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc
      961 gggtgagtca cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga
     1021 ctccacggag taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac
     1081 gtcgtcttta ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt
     1141 ggagactgaa gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt
     1201 tgagtttgga tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc
     1261 atttcaggtg tcgtgaggct agcatcgatt gatcaacaag tttgtacaaa aaagcaggct
     1321 tcgaaggaga tagaaccatg gagcggagga tgaaagccgg atacttggac cagcaagtgc
     1381 cctacacctt cagcagcaaa tcgcccggaa atgggagctt gcgcgaagcg ctgatcggcc
     1441 cgctggggaa gctcatggac ccgggctccc tgccgcccct cgactctgaa gatctcttcc
     1501 aggatctaag tcacttccag gagacgtggc tcgctgaagc tcaggtacca gacagtgatg
     1561 agcagtttgt tcctgatttc cattcagaaa acctagcttt ccacagcccc accaccagga
     1621 tcaagaagga gccccagagt ccccgcacag acccggccct gtcctgcagc aggaagccgc
     1681 cactccccta ccaccatggc gagcagtgcc tttactccag tgcctatgac ccccccagac
     1741 aaatcgccat caagtcccct gcccctggtg cccttggaca gtcgccccta cagccctttc
     1801 cccgggcaga gcaacggaat ttcctgagat cctctggcac ctcccagccc caccctggcc
     1861 atgggtacct cggggaacat agctccgtct tccagcagcc cctggacatt tgccactcct
     1921 tcacatctca gggagggggc cgggaacccc tcccagcccc ctaccaacac cagctgtcgg
     1981 agccctgccc accctatccc cagcagagct ttaagcaaga ataccatgat cccctgtatg
     2041 aacaggcggg ccagccagcc gtggaccagg gtggggtcaa tgggcacagg tacccagggg
     2101 cgggggtggt gatcaaacag gaacagacgg acttcgccta cgactcagat gtcaccgggt
     2161 gcgcatcaat gtacctccac acagagggct tctctgggcc ctctccaggt gacggggcca
     2221 tgggctatgg ctatgagaaa cctctgcgac cattcccaga tgatgtctgc gttgtccctg
     2281 agaaatttga aggagacatc aagcaggaag gggtcggtgc atttcgagag gggccgccct
     2341 accagcgccg gggtgccctg cagctgtggc aatttctggt ggccttgctg gatgacccaa
     2401 caaatgccca tttcattgcc tggacgggcc ggggaatgga gttcaagctc attgagcctg
     2461 aggaggtcgc caggctctgg ggcatccaga agaaccggcc agccatgaat tacgacaagc
     2521 tgagccgctc gctccgatac tattatgaga aaggcatcat gcagaaggtg gctggtgagc
     2581 gttacgtgta caagtttgtg tgtgagcccg aggccctctt ctctttggcc ttcccggaca
     2641 atcagcgtcc agctctcaag gctgagtttg accggcctgt cagtgaggag gacacagtcc
     2701 ctttgtccca cttggatgag agccccgcct acctcccaga gctggctggc cccgcccagc
     2761 catttggccc caagggtggc tactcttact agacccagct ttcttgtaca aagtggttga
     2821 tatcggtaag cctatcccta accctctcct cggtctcgat tctacgtagt aatgaactag
     2881 taccggttaa gtcgacaatc aacgcgttaa gtcgacaatc aacctctgga ttacaaaatt
     2941 tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct
     3001 gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt ctcctccttg
     3061 tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag gcaacgtggc
     3121 gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc caccacctgt
     3181 cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga actcatcgcc
     3241 gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa ttccgtggtg
     3301 ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac ctggattctg
     3361 cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct tccttcccgc
     3421 ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc ttcgccctca gacgagtcgg
     3481 atctcccttt gggccgcctc cccgcgtcga ctttaagacc aatgacttac aaggcagctg
     3541 tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac
     3601 gaagacaaga tctgcttttt gcttgtactg ggtctctctg gttagaccag atctgagcct
     3661 gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc ttgccttgag
     3721 tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga tccctcagac
     3781 ccttttagtc agtgtggaaa atctctagca gtacgtatag tagttcatgt catcttatta
     3841 ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg
     3901 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     3961 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc
     4021 tctagctatc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     4081 ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     4141 ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggga cgtacccaat
     4201 tcgccctata gtgagtcgta ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac
     4261 tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     4321 tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     4381 ggcgaatggg acgcgccctg tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc
     4441 agcgtgaccg ctacacttgc cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc
     4501 tttctcgcca cgttcgccgg ctttccccgt caagctctaa atcgggggct ccctttaggg
     4561 ttccgattta gtgctttacg gcacctcgac cccaaaaaac ttgattaggg tgatggttca
     4621 cgtagtgggc catcgccctg atagacggtt tttcgccctt tgacgttgga gtccacgttc
     4681 tttaatagtg gactcttgtt ccaaactgga acaacactca accctatctc ggtctattct
     4741 tttgatttat aagggatttt gccgatttcg gcctattggt taaaaaatga gctgatttaa
     4801 caaaaattta acgcgaattt taacaaaata ttaacgctta caatttaggt ggcacttttc
     4861 ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     4921 cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     4981 gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     5041 ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     5101 tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     5161 aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta
     5221 ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     5281 agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     5341 gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     5401 gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     5461 gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     5521 tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     5581 ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     5641 cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     5701 gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     5761 cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     5821 tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     5881 aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     5941 aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     6001 gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     6061 cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     6121 ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc
     6181 accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     6241 tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     6301 cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     6361 gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     6421 ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     6481 cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     6541 tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     6601 ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     6661 ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     6721 ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     6781 gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg
     6841 acaggtttcc cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca
     6901 ctcattaggc accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg
     6961 tgagcggata acaatttcac acaggaaaca gctatgacca tgattacgcc aagcgcgcaa
     7021 ttaaccctca ctaaagggaa caaaagctgg agctgcaagc ttaatgtagt cttatgcaat
     7081 actcttgtag tcttgcaaca tggtaacgat gagttagcaa catgccttac aaggagagaa
     7141 aaagcaccgt gcatgccgat tggtggaagt aaggtggtac gatcgtgcct tattaggaag
     7201 gcaacagacg ggtctgacat ggattggacg aaccactgaa ttgccgcatt gcagagatat
     7261 tgtatttaag tgcctagctc gatacataaa cgggtctctc tggttagacc agatctgagc
     7321 ctgggagctc tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg
     7381 agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag
     7441 acccttttag tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg
     7501 aaagggaaac cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca
     7561 agaggcgagg ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag
     7621 gagagagatg ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa
     7681 aaaattcggt taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca
     7741 agcagggagc tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt
     7801 agacaaatac tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca
     7861 ttatataata cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc
     7921 aaggaagctt tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa
     7981 gcggccgctg atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt
     8041 atataaatat aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag
     8101 aagagtggtg cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt
     8161 gggagcagca ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca
     8221 attattgtct ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca
     8281 gcatctgttg caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt
     8341 ggaaagatac ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat
     8401 ttgcaccact gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg
     8461 gaatcacacg acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca
     8521 ctccttaatt gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt
     8581 agataaatgg gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa
     8641 attattcata atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc
     8701 tatagtgaat agagttaggc agggatattc accattatcg tttcagaccc acctcccaac
     8761 cccgagggga cccatgcatt gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     8821 ggctccccag caggcagaag tatgcaaagc atgcgtctca attagtcagc aaccatagtc
     8881 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     8941 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     9001 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctttctaga
     9061 ggtaccacca tggccaagcc tttgtctcaa gaagaatcca ccctcattga aagagcaacg
     9121 gctacaatca acagcatccc catctctgaa gactacagcg tcgccagcgc agctctctct
     9181 agcgacggcc gcatcttcac tggtgtcaat gtatatcatt ttactggggg accttgtgca
     9241 gaactcgtgg tgctgggcac tgctgctgct gcggcagctg gcaacctgac ttgtatcgtc
     9301 gcgatcggaa atgagaacag gggcatcttg agcccctgcg gacggtgccg acaggtgctt
     9361 ctcgatctgc atcctgggat caaagccata gtgaaggaca gtgatggaca gccgacggca
     9421 gttgggattc gtgaattgct gccctctggt tatgtgtggg agggcctgca gctgcagtag
     9481 taaggcgcgc cgttaacgaa ttctagatct tgagacaaat ggcagtattc atccacaatt
     9541 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
     9601 caacagacat acaaactaaa gaattacaaa aacaaattac aaaaattcaa aattttcggg
     9661 tttattacag ggacagcaga gatccacttt ggcgcc
//
