LOCUS       sequence-142602-         576 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 576)
  TITLE     SGC 2016 plasmids
REFERENCE   2  (bases 1 to 576)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: SMARCA4A
FEATURES             Location/Qualifiers
     source          1..576
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(21..38)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(464..484)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             complement(509..526)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     RBS             537..559
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
ORIGIN
        1 ttgttagcag ccggatctca gtggtggtgg tggtggtgct cgagtgcggc cgcaagcttg
       61 tcgacggagc tcgaattcgg atccgtatcc acctttactg tcaatcatct tctttttcga
      121 ttttctggcg cacgctggta aacacgctct gcagaacaat gctatcttca taaatcaggc
      181 tgccttccag gttaaaggtc tgcgcattct ggcacagcag catcacatct ttttccagat
      241 cattcaggct acgatattta tgattgcgaa tacgttcttt aattttttta aaatccaccg
      301 gtttacgaat cagttcataa tattccggca gttctttacg gctcggcagc tgaataaaca
      361 cttcgctcag ctgacgaccg ctgctgctat ctttatattt aatcaccgca tcaacaattt
      421 ttttcatttt tttggtcagg ttcggcggat tcgggctcag catggattgg aagtacaggt
      481 tctcggtacc cagatctaca ccagaagaat gatgatgatg atggtgcata tgtatatctc
      541 cttcttaaag ttaaacaaaa ttatttctag agggga
//
