LOCUS       sequence-142714-         910 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses NET1A-V5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 910)
  AUTHORS   Schaffer BE, Levin RS, Hertz NT, Maures TJ, Schoof ML, Hollstein PE,
            Benayoun BA, Banko MR, Shaw RJ, Shokat KM, Brunet A
  TITLE     Identification of AMPK Phosphorylation Sites Reveals a Network of 
            Proteins Involved in Cell Invasion and Facilitates Large-Scale 
            Substrate Prediction.
  JOURNAL   Cell Metab. 2015 Nov 3;22(5):907-21. doi: 
            10.1016/j.cmet.2015.09.009. Epub 2015 Oct 8.
  PUBMED    26456332
REFERENCE   2  (bases 1 to 910)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2015.09.009"; journalName: "Cell Metab"; date: 
            "2015-11-3- 3"; volume: "22"; issue: "5"; pages: "907-21"
COMMENT     Sequence Label: pcDNA 3.2/V5-DEST NET1A S46A
FEATURES             Location/Qualifiers
     source          1..910
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     53..72
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        53..71
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    101..125
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 gcagagctct ctggctaact agagaaccca ctgcttactg gcttatcgaa attaatacga
       61 ctcactatag ggagacccaa gctggctagt taagctatca acaagtttgt acaaaaaagt
      121 tggcatggtg gcacatgatg agactggagg tctcctacct attaaaagga ccatacgagt
      181 cctagatgtc aataaccagt ccttcagaga acaagaggag ccaagcaata aaagagttcg
      241 acctctggct cgtgtcacgg ccttggcaaa tttaatctct cctgtaagaa atggagctgt
      301 cagacgtttt ggtcaaacaa tacagtcatt tacccttcgt ggtgaccaca gatccccagc
      361 ctctgcccag aagttttcta gcaggtcaac agtcccaaca cccgccaaga gaaggagcag
      421 tgcactgtgg tcagagatgc tggacatcac catgaaggag tctctcacca ccagggagat
      481 cagacggcag gaggcaatat atgaaatgtc ccgaggtgaa caggatttaa ttgaggatct
      541 caaacttgca agaaaggcct accatgaccc catgttaaag ttgtccatca tgtcagaaga
      601 ggaactcaca catatatttg gtgatctgga ctcttacata cctctgcatg aagatttgtt
      661 gacaagaata ggagaagcaa ccaagcctga tggaacagtg gagcagattg gtcacattct
      721 cgtgagctgg ttaccgcgct tgaatgccta cagaggttac tgtagtaacc agctggcagc
      781 caaagctctt cttgatcaaa agaaacagga tccaagagtc caagacttcc tccagcgatg
      841 tctcgagtct cccttcagtc gaaaactaga tctttggagt ttcctagata tccctcgaag
      901 tcgcctagtc
//
