LOCUS       sequence-142715-         743 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 743)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 743)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: BMP2K
FEATURES             Location/Qualifiers
     source          1..743
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             10..27
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             37..207
                     /codon_start=1
                     /product="Z domain of staphylococcal protein A, engineered 
                     to contain multiple positive charges (Hedhammar and Hober, 
                     2007)"
                     /label=Z-basic tag
                     /note="useful for purifying tagged proteins by 
                     cation-exchange chromatography"
                     /translation="VDNKFNKERRRARREIRHLPNLNREQRRAFIRSLRDDPSQSANLL
                     AEAKKLNDAQPK"
     CDS             214..234
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 atacatatgc accatcatca tcatcattct tctggtgtgg ataacaagtt caacaaggag
       61 cgtcgaagag ctcgccgtga aattcgccat ctgccgaacc tgaaccgcga acagcgtcgc
      121 gcatttattc gcagcctgcg cgatgatccg agccagagcg cgaacctgct ggcggaagcg
      181 aagaagctga acgatgcgca gccgaagggt accgagaacc tgtacttcca atccatgtcc
      241 gtgggggtcc gggtgttcgc ggtcggccgc caccaggtca ccctggaaga gtcgctggcc
      301 gaaggtggat tctccacagt tttcctcgtg cgtactcacg gtggaatccg atgtgcattg
      361 aagcgaatgt atgtcaataa catgccagac ctcaatgttt gtaaaaggga aattacaatt
      421 atgaaagagc tatctggtca caaaaatatt gtgggctatt tggactgtgc tgttaattca
      481 attagtgata atgtatggga agtccttatc ttaatggaat attgtcgagc tggacaggta
      541 gtgaatcaaa tgaataagaa gctacagacg ggttttacag aaccagaagt gttacagata
      601 ttctgtgata cctgtgaagc tgttgcaagg ttgcatcagt gtaagactcc aataattcac
      661 cgggatctga aggtagaaaa tattttgttg aatgatggtg ggaactatgt actttgtgac
      721 tttggcagtg ccactaataa att
//
