LOCUS       sequence-142717-        1012 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses NET1A-V5 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1012)
  AUTHORS   Schaffer BE, Levin RS, Hertz NT, Maures TJ, Schoof ML, Hollstein PE,
            Benayoun BA, Banko MR, Shaw RJ, Shokat KM, Brunet A
  TITLE     Identification of AMPK Phosphorylation Sites Reveals a Network of 
            Proteins Involved in Cell Invasion and Facilitates Large-Scale 
            Substrate Prediction.
  JOURNAL   Cell Metab. 2015 Nov 3;22(5):907-21. doi: 
            10.1016/j.cmet.2015.09.009. Epub 2015 Oct 8.
  PUBMED    26456332
REFERENCE   2  (bases 1 to 1012)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cmet.2015.09.009"; journalName: "Cell Metab"; date: 
            "2015-11-3- 3"; volume: "22"; issue: "5"; pages: "907-21"
COMMENT     Sequence Label: pcDNA 3.2/V5-DEST NET1A S46A
FEATURES             Location/Qualifiers
     source          1..1012
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     polyA_signal    50..98
                     /label=HSV TK poly(A) signal
                     /note="herpes simplex virus thymidine kinase 
                     polyadenylation signal (Cole and Stacy, 1985)"
     primer_bind     123..142
                     /label=TK-pA-R
                     /note="Thymidine kinase polyA, reverse primer"
     CDS             complement(182..223)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     protein_bind    249..273
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 ggtggggtat cgacagagtg ccagccctgg gaccgaaccc cgcgtttatg aacaaacgac
       61 ccaacacccg tgcgttttat tctgtctttt tattgccgtc atagcgcggg ttccttccgg
      121 tattgtctcc ttccgtgttt cagttagcct cccccgttta aactcattac taaccggtac
      181 gcgtagaatc gagaccgagg agagggttag ggataggctt accttcgaac cgcgggccct
      241 ctagatcaac cactttgtac aagaaagttg ggtacaccaa agtctctttc cgtttgccac
      301 cagaaagggc tttgtccctc gctcttcgga tgccgggctg tgtctggtgt gtctttaagc
      361 tcttgctgtc ctctgccatc tgcatgccag agccacatct gtaagcgttt tcatcaactt
      421 ctacctgagt aacactggaa actgtggatg ccctcctctg ggctgtgagt ttcctcgcag
      481 aggggtggtt cccctcacac tcttcgtgca gctccggcag gccctgcagc tcaggtggac
      541 tgcctgccga ctggaagggg gcaatggccg ctcgaataca gttgaaccac tgctgcttgt
      601 ggaacacgtc attggcttgc agagtgtgag actgggctgg agaggggtca tggaagcgaa
      661 ttctaaagat atttttagct ttctctgagt tactgaaagc tcctcgaaag gagcctccca
      721 ttctcacatc tccatcctgc aggtcttcta ggactagctc ttggactggg attggctgcc
      781 ggtaaacctg gtaagagtgc cgttcgttcc gtgtgacggg ccgagtcaga accaagatgt
      841 cttgaaacag gaaaatgtaa agtttatgtc cactcttgct ccgcagctcc ccatggcaca
      901 gcagcacttt gctcgcttcg attctggggt ccctctgctt ttcatccagg tactccagct
      961 tgtcgatgta atactggcac tcggattcac ctttcttcaa gttgatatca ga
//
