LOCUS       sequence-142722-         867 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression for structure determination; may not be full 
            ORF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 867)
  TITLE     SGC Oxford
REFERENCE   2  (bases 1 to 867)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: GYG2
FEATURES             Location/Qualifiers
     source          1..867
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(17..34)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(84..107)
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             complement(108..125)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(126..146)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
ORIGIN
        1 tagcagccgg atctcagtgg tggtggtggt ggtgctcgag tgcggccgca agcttgtcga
       61 cggagctcga attcggatcc tcacttatcg tcgtcatcct tgtaatcatg gtggtgatga
      121 tggtgcgatt ggaagtagag gttctctgct tggacgcttt tataaagggg cagcacgttg
      181 ttctggtaga ccgtccacca gagatgaagg aatgccgcct ggtgctggct gctggacact
      241 gagccttgct ccaacaccga gccactctgt ggattgtact tgtagttcca aggtttcatg
      301 gaccccaaaa agtggacgac ctttgcactg gaaccgaatt gcttgaaggc agggctgtaa
      361 gtgtacatcg tgttactact caagttatag atgaacggca ggtgcttgtg gatgtctgtg
      421 gtcgaccagt tcctgaagaa actattcagt aagccttggt ctgccccgtc aaagctgccg
      481 tgttccatgg cgtgctgtag caggagttta tgcgtgtgga gagaaggctg gaagacaaac
      541 accccgctat tgaagcaatc cggccatccg gggtccgggg ccgcagaaaa ctctcccctg
      601 tcaaacagct catcgacatt ggacagcacc agagtgtctg catccaggaa gacacacttg
      661 ctgtagtgag tgagagtcca acagtgaagc ttggtgaggg tgagcccgag ctcaggtctc
      721 ttcagaaagg ccaggtggat gtagtcggca ctatcgatta gattcacttc aatgacttca
      781 tcgaacacct tcgagaggat gcgcctgagc agatcggaca cctgaggagt gatcaacacc
      841 accagcttcc tcgtcagcct gtgtctc
//
