LOCUS       sequence-142732-         962 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  a plasmid derived from pKHR5: in addition to Venus reporter gene, it
            also contains cmlc2::mCherry (red heart) reporter gene at 3' end of 
            the mcs as pKHR7. Genbank accession number is KU144825..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 962)
  AUTHORS   Hoshijima K, Jurynec MJ, Grunwald DJ
  TITLE     Precise Editing of the Zebrafish Genome Made Simple and Efficient.
  JOURNAL   Dev Cell. 2016 Mar 21;36(6):654-67. doi: 
            10.1016/j.devcel.2016.02.015.
  PUBMED    27003937
REFERENCE   2  (bases 1 to 962)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.devcel.2016.02"; journalName: "Dev Cell"; date: 
            "2016-03-21- 21"; volume: "36"; issue: "6"; pages: "654-67"
COMMENT     Sequence Label: pKHR8
FEATURES             Location/Qualifiers
     source          1..962
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     2..21
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        2..20
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     102..118
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     102..118
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     protein_bind    138..171
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     polyA_signal    235..369
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(272..291)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     326..345
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    406..613
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     602..619
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     CDS             complement(640..891)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKANFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(682..703)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 gtaatacgac tcactatagg gcgaattgga gctccaccgc ggtggcggcc aggtccttta
       61 gtcgggtatt cgggtaggga taacagggta atggcggccg ctctagaact agtggatccc
      121 ccgggctgca ggaattcgaa gttcctattc tctagaaagt ataggaactt cttaaaaaac
      181 ctcccacacc tccccctgaa cctgaaacat aaaatgaatg caattgttgt tgttaacttg
      241 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
      301 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
      361 gtctggatca tcatcgatgg tacccaccgc ggtggcggcg atctctcccc agcatgcctg
      421 ctattgtctt cccaatcctc ccccttgctg tcctgcccca ccccaccccc cagaatagaa
      481 tgacacctac tcagacaatg cgatgcaatt tcctcatttt attaggaaag gacagtggga
      541 gtggcacctt ccagggtcaa ggaaggcacg ggggaggggc aaacaacaga tggctggcaa
      601 ctagaaggca cagtcgaggc tgatcagcgg gccgctttac ttgtacagct cgtccatgcc
      661 gagagtgatc ccggcggcgg tcacgaactc cagcaggacc atgtgatcgc gcttctcgtt
      721 ggggtctttg ctcagggcgg actggtagct caggtagtgg ttgtcgggca gcagcacggg
      781 gccgtcgccg atgggggtgt tctgctggta gtggtcggcg agctgcacgc cgccgtcctc
      841 gatgttgtgg cggatcttga agttggcctt gatgccgttc ttctgcttgt cggcggtgat
      901 atagacgttg tggctgttgt agttgtactc cagcttgtgc cccaggatgt tgccgtcctc
      961 ct
//
