LOCUS       sequence-142796-         703 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Contains GFPmut3b under the Ptrc promoter. Suicide vector inserts 
            into slr0168 site of Synechocystis. Carries chloramphenicol 
            resistance. Propogates in E. coli.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 703)
  AUTHORS   Yao L, Cengic I, Anfelt J, Hudson EP
  TITLE     Multiple Gene Repression in Cyanobacteria Using CRISPRi.
  JOURNAL   ACS Synth Biol. 2015 Dec 28.
  PUBMED    26689101
REFERENCE   2  (bases 1 to 703)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2015 Dec 28."
COMMENT     Sequence Label: pMD19T-slr0168-Ptrc-GFP3mut-CmR
FEATURES             Location/Qualifiers
     source          1..703
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(38..66)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     661..681
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
ORIGIN
        1 aaactagatg cgtaaaggag aagaactttt cactggagtt gtcccaattc ttgttgaatt
       61 agatggtgat gttaatgggc acaaattttc tgtcagtgga gagggtgaag gtgatgcaac
      121 atacggaaaa cttaccctta aatttatttg cactactgga aaactacctg ttccatggcc
      181 aacacttgtc actactttcg gttatggtgt tcaatgcttt gcgagatacc cagatcatat
      241 gaaacagcat gactttttca agagtgccat gcccgaaggt tatgtacagg aaagaactat
      301 atttttcaaa gatgacggga actacaagac acgtgctgaa gtcaagtttg aaggtgatac
      361 ccttgttaat agaatcgagt taaaaggtat tgattttaaa gaagatggaa acattcttgg
      421 acacaaattg gaatacaact ataactcaca caatgtatac atcatggcag acaaacaaaa
      481 gaatggaatc aaagttaact tcaaaattag acacaacatt gaagatggaa gcgttcaact
      541 agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt taccagacaa
      601 ccattacctg tccacacaat ctgccctttc gaaagatccc aacgaaaaga gagaccacat
      661 ggtccttctt gagtttgtaa cagctgctgg gattacacat ggc
//
