LOCUS       sequence-14383-e        7729 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7729)
  AUTHORS   Tabor JJ, Levskaya A, Voigt CA
  TITLE     Multichromatic control of gene expression in Escherichia coli.
  JOURNAL   J Mol Biol. 2011 Jan 14. 405(2):315-24.
  PUBMED    21035461
REFERENCE   2  (bases 1 to 7729)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Mol Biol.
            2011 Jan 14. 405(2):315-24."
COMMENT     Sequence Label: pJT106b
FEATURES             Location/Qualifiers
     source          1..7729
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     RBS             106..117
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     CDS             138..3182
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /label=lacZ
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGSLSQMWIGDKNQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAEFQLSAGRYHYQLVWCQK"
     primer_bind     complement(140..162)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     terminator      3217..3288
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      3304..3331
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     CDS             3479..4189
                     /codon_start=1
                     /gene="cI"
                     /product="phage lambda repressor"
                     /label=lambda repressor
                     /translation="MSTKKKPLTQEQLEDARRLKAIYEKKKNELGLSQESVADKMGMGQ
                     SGVGALFNGINALNAYNAALLAKILKVSVEEFSPSIAREIYEMYEAVSMQPSLRSEYEY
                     PVFSHVQAGMFSPELRTFTKGDAERWVSTTKKASDSAFWLEVEGNSMTAPTGSKPSFPD
                     GMLILVDPEQAVEPGDFCIARLGGDEFTFKKLIRDSGQVFLQPLNPQYPMIPCNESCSV
                     VGKVIASQWPEETFG"
     CDS             4190..4222
                     /codon_start=1
                     /product="C-terminal peptide that mediates degradation in 
                     bacteria through the ClpXP and ClpAP proteases (McGinness 
                     et al., 2006)"
                     /label=ssrA tag (LVA)
                     /note="mutant LVA variant that confers accelerated 
                     degradation under some conditions (Andersen et al., 1998)"
                     /translation="AANDENYALVA"
     terminator      4270..4341
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      4357..4384
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
     misc_feature    4391..4411
                     /label=BioBrick suffix
                     /note="universal suffix for all parts"
     primer_bind     complement(4495..4512)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     CDS             complement(4774..5724)
                     /codon_start=1
                     /gene="rep101"
                     /product="RepA protein needed for replication with the 
                     pSC101 origin"
                     /label=Rep101
                     /translation="MSELVVFKANELAISRYDLTEHETKLILCCVALLNPTIENPTRKE
                     RTVSFTYNQYAQMMNISRENAYGVLAKATRELMTRTVEIRNPLVKGFEIFQWTNYAKFS
                     SEKLELVFSEEILPYLFQLKKFIKYNLEHVKSFENKYSMRIYEWLLKELTQKKTHKANI
                     EISLDEFKFMLMLENNYHEFKRLNQWVLKPISKDLNTYSNMKLVVDKRGRPTDTLIFQV
                     ELDRQMDLVTELENNQIKMNGDKIPTTITSDSYLRNGLRKTLHDALTAKIQLTSFEAKF
                     LSDMQSKHDLNGSFSWLTQKQRTTLENILAKYGRI"
     rep_origin      5772..5994
                     /label=pSC101 ori
                     /note="low-copy replication origin that requires the Rep101
                     protein"
     terminator      complement(6486..6580)
                     /label=lambda t0 terminator
                     /note="transcription terminator from phage lambda"
     CDS             complement(join(6610..7401,7402..7470))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7233..7252
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7471..7575)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7640..7658
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     terminator      complement(7662..7705)
                     /label=bacterial terminator
                     /note="putative bacterial transcription terminator"
     misc_feature    7708..7729
                     /label=BioBrick prefix
                     /note="BioBrick prefix for parts that do not start with 
                     ""ATG"""
ORIGIN
        1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac
       61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tggagaaaga ggagaaatac
      121 tagagatgat agatcccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
      181 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
      241 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt
      301 ttccggcacc agaagcggtg ccggaaagct ggctggagtg cgatcttcct gaggccgata
      361 ctgtcgtcgt cccctcaaac tggcagatgc acggttacga tgcgcccatc tacaccaacg
      421 tgacctatcc cattacggtc aatccgccgt ttgttcccac ggagaatccg acgggttgtt
      481 actcgctcac atttaatgtt gatgaaagct ggctacagga aggccagacg cgaattattt
      541 ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg gcgctgggtc ggttacggcc
      601 aggacagtcg tttgccgtct gaatttgacc tgagcgcatt tttacgcgcc ggagaaaacc
      661 gcctcgcggt gatggtgctg cgctggagtg acggcagtta tctggaagat caggatatgt
      721 ggcggatgag cggcattttc cgtgacgtct cgttgctgca taaaccgact acacaaatca
      781 gcgatttcca tgttgccact cgctttaatg atgatttcag ccgcgctgta ctggaggctg
      841 aagttcagat gtgcggcgag ttgcgtgact acctacgggt aacagtttct ttatggcagg
      901 gtgaaacgca ggtcgccagc ggcaccgcgc ctttcggcgg tgaaattatc gatgagcgtg
      961 gtggttatgc cgatcgcgtc acactacgtc tgaacgtcga aaacccgaaa ctgtggagcg
     1021 ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca caccgccgac ggcacgctga
     1081 ttgaagcaga agcctgcgat gtcggtttcc gcgaggtgcg gattgaaaat ggtctgctgc
     1141 tgctgaacgg caagccgttg ctgattcgag gcgttaaccg tcacgagcat catcctctgc
     1201 atggtcaggt catggatgag cagacgatgg tgcaggatat cctgctgatg aagcagaaca
     1261 actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc gctgtggtac acgctgtgcg
     1321 accgctacgg cctgtatgtg gtggatgaag ccaatattga aacccacggc atggtgccaa
     1381 tgaatcgtct gaccgatgat ccgcgctggc taccggcgat gagcgaacgc gtaacgcgaa
     1441 tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg gtcgctgggg aatgaatcag
     1501 gccacggcgc taatcacgac gcgctgtatc gctggatcaa atctgtcgat ccttcccgcc
     1561 cggtgcagta tgaaggcggc ggagccgaca ccacggccac cgatattatt tgcccgatgt
     1621 acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc gaaatggtcc atcaaaaaat
     1681 ggctttcgct acctggagag acgcgcccgc tgatcctttg cgaatacgcc cacgcgatgg
     1741 gtaacagtct tggcggtttc gctaaatact ggcaggcgtt tcgtcagtat ccccgtttac
     1801 agggcggctt cgtctgggac tgggtggatc agtcgctgat taaatatgat gaaaacggca
     1861 acccgtggtc ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc cagttctgta
     1921 tgaacggtct ggtctttgcc gaccgcacgc cgcatccagc gctgacggaa gcaaaacacc
     1981 agcagcagtt tttccagttc cgtttatccg ggcaaaccat cgaagtgacc agcgaatacc
     2041 tgttccgtca tagcgataac gagctcctgc actggatggt ggcgctggat ggtaagccgc
     2101 tggcaagcgg tgaagtgcct ctggatgtcg ctccacaagg taaacagttg attgaactgc
     2161 ctgaactacc gcagccggag agcgccgggc aactctggct cacagtacgc gtagtgcaac
     2221 cgaacgcgac cgcatggtca gaagccgggc acatcagcgc ctggcagcag tggcgtctgg
     2281 cggaaaacct cagtgtgacg ctccccgccg cgtcccacgc catcccgcat ctgaccacca
     2341 gcgaaatgga tttttgcatc gagctgggta ataagcgttg gcaatttaac cgccagtcag
     2401 gctctctttc acagatgtgg attggcgata aaaaccaact gctgacgccg ctgcgcgatc
     2461 agttcacccg tgcaccgctg gataacgaca ttggcgtaag tgaagcgacc cgcattgacc
     2521 ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta ccaggccgaa gcagcgttgt
     2581 tgcagtgcac ggcagataca cttgctgatg cggtgctgat tacgaccgct cacgcgtggc
     2641 agcatcaggg gaaaacctta tttatcagcc ggaaaaccta ccggattgat ggtagtggtc
     2701 aaatggcgat taccgttgat gttgaagtgg cgagcgatac accgcatccg gcgcggattg
     2761 gcctgaactg ccagctggcg caggtagcag agcgggtaaa ctggctcgga ttagggccgc
     2821 aagaaaacta tcccgaccgc cttactgccg cctgttttga ccgctgggat ctgccattgt
     2881 cagacatgta taccccgtac gtcttcccga gcgaaaacgg tctgcgctgc gggacgcgcg
     2941 aattgaatta tggcccacac cagtggcgcg gcgacttcca gttcaacatc agccgctaca
     3001 gtcaacagca actgatggaa accagccatc gccatctgct gcacgcggaa gaaggcacat
     3061 ggctgaatat cgacggtttc catatgggga ttggtggcga cgactcctgg agcccgtcag
     3121 tatcggcgga attccagctg agcgccggtc gctaccatta ccagttggtc tggtgtcaaa
     3181 aagcggccgc tcgagtctaa tactagagcc aggcatcaaa taaaacgaaa ggctcagtcg
     3241 aaagactggg cctttcgttt tatctgttgt ttgtcggtga acgctctcta ctagagtcac
     3301 actggctcac cttcgggtgg gcctttctgc gtttatatac tagagtccct tgcatttaca
     3361 ttttgaaaca tctatagcga taaatgaaac atcttaaaag ttttagtatc atattcgtgt
     3421 tggattattc tgcatttttg gggagaatgg acttactaga gtcacacagg acggccggat
     3481 gagcacaaaa aagaaaccat taacacaaga gcagcttgag gacgcacgtc gccttaaagc
     3541 aatttatgaa aaaaagaaaa atgaacttgg cttatcccag gaatctgtcg cagacaagat
     3601 ggggatgggg cagtcaggcg ttggtgcttt atttaatggc atcaatgcat taaatgctta
     3661 taacgccgca ttgcttgcaa aaattctcaa agttagcgtt gaagaattta gcccttcaat
     3721 cgccagagaa atctacgaga tgtatgaagc ggttagtatg cagccgtcac ttagaagtga
     3781 gtatgagtac cctgtttttt ctcatgttca ggcagggatg ttctcacctg agcttagaac
     3841 ctttaccaaa ggtgatgcgg agagatgggt aagcacaacc aaaaaagcca gtgattctgc
     3901 attctggctt gaggttgaag gtaattccat gaccgcacca acaggctcca agccaagctt
     3961 tcctgacgga atgttaattc tcgttgaccc tgagcaggct gttgagccag gtgatttctg
     4021 catagccaga cttgggggtg atgagtttac cttcaagaaa ctgatcaggg atagcggtca
     4081 ggtgttttta caaccactaa acccacagta cccaatgatc ccatgcaatg agagttgttc
     4141 cgttgtgggg aaagttatcg ctagtcagtg gcctgaagag acgtttggcg ctgcaaacga
     4201 cgaaaactac gctttagtag cttaataaca ctgatagtgc tagtgtagat cactactaga
     4261 gccaggcatc aaataaaacg aaaggctcag tcgaaagact gggcctttcg ttttatctgt
     4321 tgtttgtcgg tgaacgctct ctactagagt cacactggct caccttcggg tgggcctttc
     4381 tgcgtttata tactagtagc ggccgctgca gtccggcaaa aaaacgggca aggtgtcacc
     4441 accctgccct ttttctttaa aaccgaaaag attacttcgc gttatgcagg cttcctcgct
     4501 cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc
     4561 ggtaatctcg aggttacatt gtcgatctgt tcatggtgaa cagctttaaa tgcaccaaaa
     4621 actcgtaaaa gctctgatgt atctatcttt tttacaccgt tttcatctgt gcatatggac
     4681 agttttccct ttgatatcta acggtgaaca gttgttctac ttttgtttgt tagtcttgat
     4741 gcttcactga tagatacaag agccataaga acctcagatc cttccgtatt tagccagtat
     4801 gttctctagt gtggttcgtt gtttttgcgt gagccatgag aacgaaccat tgagatcatg
     4861 cttactttgc atgtcactca aaaattttgc ctcaaaactg gtgagctgaa tttttgcagt
     4921 taaagcatcg tgtagtgttt ttcttagtcc gttacgtagg taggaatctg atgtaatggt
     4981 tgttggtatt ttgtcaccat tcatttttat ctggttgttc tcaagttcgg ttacgagatc
     5041 catttgtcta tctagttcaa cttggaaaat caacgtatca gtcgggcggc ctcgcttatc
     5101 aaccaccaat ttcatattgc tgtaagtgtt taaatcttta cttattggtt tcaaaaccca
     5161 ttggttaagc cttttaaact catggtagtt attttcaagc attaacatga acttaaattc
     5221 atcaaggcta atctctatat ttgccttgtg agttttcttt tgtgttagtt cttttaataa
     5281 ccactcataa atcctcatag agtatttgtt ttcaaaagac ttaacatgtt ccagattata
     5341 ttttatgaat ttttttaact ggaaaagata aggcaatatc tcttcactaa aaactaattc
     5401 taatttttcg cttgagaact tggcatagtt tgtccactgg aaaatctcaa agcctttaac
     5461 caaaggattc ctgatttcca cagttctcgt catcagctct ctggttgctt tagctaatac
     5521 accataagca ttttccctac tgatgttcat catctgagcg tattggttat aagtgaacga
     5581 taccgtccgt tctttccttg tagggttttc aatcgtgggg ttgagtagtg ccacacagca
     5641 taaaattagc ttggtttcat gctccgttaa gtcatagcga ctaatcgcta gttcatttgc
     5701 tttgaaaaca actaattcag acatacatct caattggtct aggtgatttt aatcactata
     5761 ccaattgaga tgggctagtc aatgataatt acatgtcctt ttcctttgag ttgtgggtat
     5821 ctgtaaattc tgctagacct ttgctggaaa acttgtaaat tctgctagac cctctgtaaa
     5881 ttccgctaga cctttgtgtg ttttttttgt ttatattcaa gtggttataa tttatagaat
     5941 aaagaaagaa taaaaaaaga taaaaagaat agatcccagc cctgtgtata actcactact
     6001 ttagtcagtt ccgcagtatt acaaaaggat gtcgcaaacg ctgtttgctc ctctacaaaa
     6061 cagaccttaa aaccctaaag gcttaagtag caccctcgca agctcgggca aatcgctgaa
     6121 tattcctttt gtctccgacc atcaggcacc tgagtcgctg tctttttcgt gacattcagt
     6181 tcgctgcgct cacggctctg gcagtgaatg ggggtaaatg gcactacagg cgccttttat
     6241 ggattcatgc aaggaaacta cccataatac aagaaaagcc cgtcacgggc ttctcagggc
     6301 gttttatggc gggtctgcta tgtggtgcta tctgactttt tgctgttcag cagttcctgc
     6361 cctctgattt tccagtctga ccacttcgga ttatcccgtg acaggtcatt cagactggct
     6421 aatgcaccca gtaaggcagc ggtatcatca acaggcttac ccgtcttact gtccctagtg
     6481 cttggattct caccaataaa aaacgcccgg cggcaaccga gcgttctgaa caaatccaga
     6541 tggagttctg aggtcattac tggatctatc aacaggagtc caagcgagct cgtaaacttg
     6601 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
     6661 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
     6721 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
     6781 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
     6841 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     6901 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     6961 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     7021 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     7081 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     7141 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     7201 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     7261 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
     7321 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
     7381 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
     7441 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
     7501 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
     7561 aataggggtt ccgcgcacat ttcccatggt gccacctgac gtctaagaaa ccattattat
     7621 catgacatta acctataaaa ataggcgtat cacgaggcag aatttcagat aaaaaaaatc
     7681 cttagctttc gctaaggatg atttctggaa ttcgcggccg cttctagag
//
