LOCUS       sequence-144-exp        3634 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3634)
  AUTHORS   Fire A et al
  TITLE     Fire Lab C. Elegans Vector Kit 1997
REFERENCE   2  (bases 1 to 3634)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: L3465
FEATURES             Location/Qualifiers
     source          1..3634
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(122..150)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     898..918
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     primer_bind     complement(1023..1040)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1023..1039)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1032..1054)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(1248..1267)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     1367..1389
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(1427..1445)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        1513..1617
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1618..1686,1687..2478)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(1836..1855)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      2649..3237
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3138..3157
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3391..3408
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3525..3546
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3561..3566,3567..3584,3585..3591)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3599..3615
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3604..3626
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     join(3623..3634,1..5)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     join(3623..3634,1..5)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 atgaccatga ttacgccaag ctttacatgt cccattaatg acgtcccggg atgacgcgtc
       61 gccatgggga attcaagtac cggtagaaaa aatgagtaaa ggagaagaac ttttcactgg
      121 agttgtccca attcttgttg aattagatgg tgatgttaat gggcacaaat tttctgtcag
      181 tggagagggt gaaggtgatg caacatacgg aaaacttacc cttaaattta tttgcactac
      241 tggaaaacta cctgttccat gggtaagttt aaacatatat atactaacta accctgatta
      301 tttaaatttt cagccaacac ttgtcactac tctcacttat ggtgttcaat gcttctcgag
      361 atacccagat catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt
      421 acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgta agtttaaaca
      481 gttcggtact aactaaccat acatatttaa attttcaggt gctgaagtca agtttgaagg
      541 tgataccctt gttaatagaa tcgagttaaa aggtattgat tttaaagaag atggaaacat
      601 tcttggacac aaattggaat acaactataa ctcacacaat gtatacatca tggcagacaa
      661 acaaaagaat ggaatcaaag ttgtaagttt aaacatgatt ttactaacta actaatctga
      721 tttaaatttt cagaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
      781 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
      841 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt
      901 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggacgaac tatacaaaag
      961 cccacaagct ttacatgtcc cattaatgac gtcccgggat gacgcgtcgc catggggaat
     1021 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     1081 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     1141 cgcccttccc aacagttgcg cagcctgaat ggcgaatggc gcctgatgcg gtattttctc
     1201 cttacgcatc tgtgcggtat ttcacaccgc atatggtgca ctctcagtac aatctgctct
     1261 gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc gccctgacgg
     1321 gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg gagctgcatg
     1381 tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct cgtgatacgc
     1441 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     1501 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     1561 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     1621 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     1681 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     1741 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     1801 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     1861 attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     1921 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     1981 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     2041 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     2101 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     2161 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     2221 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     2281 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     2341 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     2401 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     2461 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     2521 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     2581 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     2641 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     2701 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     2761 actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     2821 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     2881 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     2941 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     3001 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     3061 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     3121 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     3181 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     3241 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     3301 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     3361 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     3421 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagctggcac
     3481 gacaggtttc ccgactggaa agcgggcagt gagcgcaacg caattaatgt gagttagctc
     3541 actcattagg caccccaggc tttacacttt atgcttccgg ctcgtatgtt gtgtggaatt
     3601 gtgagcggat aacaatttca cacaggaaac agct
//
