LOCUS       sequence-145559-         980 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shRNA(2) against CDK4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 980)
  AUTHORS   Acevedo M, Vernier M, Mignacca L, Lessard F, Huot G, Moiseeva O, 
            Bourdeau V, Ferbeyre G
  TITLE     A CDK4/6-Dependent Epigenetic Mechanism Protects Cancer Cells from 
            PML-induced Senescence.
  JOURNAL   Cancer Res. 2016 Jun 1;76(11):3252-64. doi: 
            10.1158/0008-5472.CAN-15-2347. Epub 2016 Mar 29.
  PUBMED    27206849
REFERENCE   2  (bases 1 to 980)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/0008-5472.CAN-15-2347"; journalName: "Cancer Res"; date: 
            "2016-06-1- 1"; volume: "76"; issue: "11"; pages: "3252-64"
COMMENT     Sequence Label: shCDK4(2)
FEATURES             Location/Qualifiers
     source          1..980
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           64..185
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           213..227
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           255..381
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     promoter        395..894
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(430..453)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     827..846
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     primer_bind     complement(915..934)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
ORIGIN
        1 gccctcactc cttctctagg cgccggaatt agatctctcg actagggata acagggtaat
       61 tgtttgaatg aggcttcagt actttacaga atcgttgcct gcacatcttg gaaacacttg
      121 ctgggattac ttcttcaggt taacccaaca gaaggctcga gaaggtatat tgctgttgac
      181 agtgagcgag cacttacacc cgtggttgtt tagtgaagcc acagatgtaa acaaccacgg
      241 gtgtaagtgc ctgcctactg cctcggaatt caaggggcta ctttaggagc aattatcttg
      301 tttactaaaa ctgaatacct tgctatctct ttgatacatt tttacaaagc tgaattaaaa
      361 tggtataaat taaatcactt ttttcaattc taccgggtag gggaggcgct tttcccaagg
      421 cagtctggag catgcgcttt agcagccccg ctgggcactt ggcgctacac aagtggcctc
      481 tggcctcgca cacattccac atccaccggt aggcgccaac cggctccgtt ctttggtggc
      541 cccttcgcgc caccttctac tcctccccta gtcaggaagt tcccccccgc cccgcagctc
      601 gcgtcgtgca ggacgtgaca aatggaagta gcacgtctca ctagtctcgt gcagatggac
      661 agcaccgctg agcaatggaa gcgggtaggc ctttggggca gcggccaata gcagctttgc
      721 tccttcgctt tctgggctca gaggctggga aggggtgggt ccgggggcgg gctcaggggc
      781 gggctcaggg gcggggcggg cgcccgaagg tcctccggag gcccggcatt ctgcacgctt
      841 caaaagcgca cgtctgccgc gctgttctcc tcttcctcat ctccgggcct ttcgacctgc
      901 agcccaagct taccatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
      961 tccccagggc cgtacgcacc
//
