LOCUS       sequence-145560-         578 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  shRNA(2) against CDK4.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 578)
  AUTHORS   Acevedo M, Vernier M, Mignacca L, Lessard F, Huot G, Moiseeva O, 
            Bourdeau V, Ferbeyre G
  TITLE     A CDK4/6-Dependent Epigenetic Mechanism Protects Cancer Cells from 
            PML-induced Senescence.
  JOURNAL   Cancer Res. 2016 Jun 1;76(11):3252-64. doi: 
            10.1158/0008-5472.CAN-15-2347. Epub 2016 Mar 29.
  PUBMED    27206849
REFERENCE   2  (bases 1 to 578)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/0008-5472.CAN-15-2347"; journalName: "Cancer Res"; date: 
            "2016-06-1- 1"; volume: "76"; issue: "11"; pages: "3252-64"
COMMENT     Sequence Label: shCDK4(2)
FEATURES             Location/Qualifiers
     source          1..578
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     ncRNA           complement(12..138)
                     /label=3' miR-30a
                     /note="sequence downstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(166..180)
                     /label=miR-30a loop
                     /note="loop from the the 71-nt precursor of the human 
                     miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     ncRNA           complement(208..329)
                     /label=5' miR-30a
                     /note="sequence upstream of the 71-nt precursor of the 
                     human miR-30a microRNA (Zeng et al., 2002)"
                     /ncRNA_class="miRNA"
     primer_bind     complement(385..401)
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     primer_bind     complement(417..439)
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
ORIGIN
        1 tagaattgaa aaaagtgatt taatttatac cattttaatt cagctttgta aaaatgtatc
       61 aaagagatag caaggtattc agttttagta aacaagataa ttgctcctaa agtagcccct
      121 tgaattccga ggcagtaggc aggcacttac acccgtggtt gtttacatct gtggcttcac
      181 taaacaacca cgggtgtaag tgctcgctca ctgtcaacag caatatacct tctcgagcct
      241 tctgttgggt taacctgaag aagtaatccc agcaagtgtt tccaagatgt gcaggcaacg
      301 attctgtaaa gtactgaagc ctcattcaaa caattaccct gttatcccta gtcgagagat
      361 ctaattccgg cgcctagaga aggagtgagg gctggataaa gggaggatcg aggcggggtc
      421 gaacgaggag gttcaagggg gagagacggg gcggatggag gaagaggagg cggaggctta
      481 gggtgtacaa agggcttgac ccagggaggg gggtcaaaag ccaaggcttc ccaggtcacg
      541 atgtagggga cctggtctgg gtgtccatgc gggccagg
//
