LOCUS       sequence-145687-         822 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dCas9-2xNLS-EGFP in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 822)
  AUTHORS   Nelles DA, Fang MY, O'Connell MR, Xu JL, Markmiller SJ, Doudna JA, 
            Yeo GW
  TITLE     Programmable RNA Tracking in Live Cells with CRISPR/Cas9.
  JOURNAL   Cell. 2016 Mar 16. pii: S0092-8674(16)30204-5. doi: 
            10.1016/j.cell.2016.02.054.
  PUBMED    26997482
REFERENCE   2  (bases 1 to 822)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2016 
            Mar 16. pii: S0092-8674(16)30204-5. doi: 
            10.1016/j.cell.2016.02.054."
COMMENT     Sequence Label: pCDNA3.1- dCas9-2xNLS-EGFP
FEATURES             Location/Qualifiers
     source          1..822
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     28..47
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        28..46
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 acccactgct tactggctta tcgaaattaa tacgactcac tatagggaga cccaagctgg
       61 ctagcgttta aacgggccct ctagactcga gcggccgcca ctgtgctgga tatctgcaga
      121 gaaccatgga caagaagtac agcatcggcc tggccatcgg caccaactct gtgggctggg
      181 ccgtgatcac cgacgagtac aaggtgccca gcaagaaatt caaggtgctg ggcaacaccg
      241 accggcacag catcaagaag aacctgatcg gcgccctgct gttcgacagc ggagaaacag
      301 ccgaggccac ccggctgaag agaaccgcca gaagaagata caccagacgg aagaaccgga
      361 tctgctatct gcaagagatc ttcagcaacg agatggccaa ggtggacgac agcttcttcc
      421 acagactgga agagtccttc ctggtggaag aggataagaa gcacgagcgg caccccatct
      481 tcggcaacat cgtggacgag gtggcctacc acgagaagta ccccaccatc taccacctga
      541 gaaagaaact ggtggacagc accgacaagg ccgacctgcg gctgatctat ctggccctgg
      601 cccacatgat caagttccgg ggccacttcc tgatcgaggg cgacctgaac cccgacaaca
      661 gcgacgtgga caagctgttc atccagctgg tgcagaccta caaccagctg ttcgaggaaa
      721 accccatcaa cgccagcggc gtggacgcca aggccatcct gtctgccaga ctgagcaaga
      781 gcagacggct ggaaaatctg atcgcccagc tgcccggcga ga
//
