LOCUS       sequence-145712-         805 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Localization: Cytosolic FRET, Excitation: , Emission: .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 805)
  AUTHORS   Ai HW, Hazelwood KL, Davidson MW, Campbell RE
  TITLE     Fluorescent protein FRET pairs for ratiometric imaging of dual 
            biosensors.
  JOURNAL   Nat Methods. 2008 May;5(5):401-3. doi: 10.1038/nmeth.1207.
  PUBMED    18425137
REFERENCE   2  (bases 1 to 805)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2008-05"; volume: "5"; issue: 
            "5"; pages: "401-3"
COMMENT     Sequence Label: mAmetrine-tdTomato-FRET
FEATURES             Location/Qualifiers
     source          1..805
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(59..61,62..64,65..775)
                     /codon_start=1
                     /product="yellow fluorescent protein with a large Stokes 
                     shift, excitable with violet light (Ai et al., 2008)"
                     /label=mAmetrine
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTL
                     KFICTSGKLPVPWPTLVTTLSYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDD
                     GSYRTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNMNVWDAYITADKQKNGIK
                     ANFKIEHNVEDGGVQLADAYQQNTPIGDGSVLLPDNHYLSFQSKLFKDPNEQRDHMVLL
                     EFVTAAGITPGMDELYK"
     primer_bind     complement(104..125)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ccgtcagatc cgctagcgct accggactca gatctcgagc tcaagcttcg aattcaccat
       61 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atcctggtcg agctggacgg
      121 cgacgtaaac ggccacaagt tcagcgtgcg cggcgagggc gagggcgatg ccaccaacgg
      181 caagctgacc ctgaagttca tctgcacctc cggcaagctg cccgtgccct ggcccaccct
      241 cgtgaccacc ctgtcttacg gcgtgcagtg cttcgcccgc taccccgacc acatgaagca
      301 gcacgacttc ttcaagtccg ccatgcccga aggctacgtc caggagcgca ccatctcctt
      361 caaggacgac ggcagctaca ggacccgcgc cgaggtgaag ttcgagggcg acaccctggt
      421 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
      481 gctggagtac aacatgaacg tgtgggacgc gtatatcacg gccgacaagc agaagaacgg
      541 catcaaagcg aacttcaaga tcgagcacaa cgtcgaggac ggcggcgtgc agctcgccga
      601 cgcgtaccag cagaacaccc ccatcggcga cggctccgtg ctgctgcctg acaaccacta
      661 cctgagcttc cagagcaagc tgttcaaaga ccccaacgag cagcgcgatc acatggtcct
      721 gctggagttc gttaccgccg ccgggatcac tcccggcatg gacgagctgt acaagtccgg
      781 actcagatcc ccaccggtcg ccacc
//
