LOCUS       sequence-145908-         825 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Over-expression of human ESRRB.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 825)
  AUTHORS   Takashima Y, Guo G, Loos R, Nichols J, Ficz G, Krueger F, Oxley D, 
            Santos F, Clarke J, Mansfield W, Reik W, Bertone P, Smith A
  TITLE     Resetting Transcription Factor Control Circuitry toward Ground-State
            Pluripotency in Human.
  JOURNAL   Cell. 2014 Sep 11;158(6):1254-69. doi: 10.1016/j.cell.2014.08.029.
  PUBMED    25215486
REFERENCE   2  (bases 1 to 825)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2014.08"; journalName: "Cell"; date: "2014-09-11- 
            11"; volume: "158"; issue: "6"; pages: "1254-69"
COMMENT     Sequence Label: pPB-PGK-hESRRB
FEATURES             Location/Qualifiers
     source          1..825
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    26..47
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(62..67,68..85,86..92)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    100..116
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     105..127
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     124..140
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     124..140
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     protein_bind    161..185
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
ORIGIN
        1 aagcgggcag tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg
       61 ctttacactt tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc
      121 acacaggaaa cagctatgac catgattacg ccaagctatc accactttgt acaagaaagc
      181 tgggtattac atggtgagcc agagatgctt tccctggcct cctggtgtcc tagtgctggg
      241 tgagatctgc ctgcctctct cattgagggg ggttggacta gaagggcgtg gattggggat
      301 ccccggggag ttgctctgat ccctgcttgt gaaggcagct gattggaagg ggcattttcc
      361 atcatggctt gacattcttt catccttggg agatcctcta agctgctctt ggccaacctt
      421 ggcctccagc atctccagga agagtttgtg catgggcact ttgccctgca gtttgacgct
      481 atagaagtgc tgcacggcct tggcggccgt ctgccgcagc agcggcagtg tcagcagcag
      541 cttgcccgtc ctccaggact cctcatggcg ctggctcagc tcgtagtcct gcagtgcctc
      601 gtgcagcagg tcctgcagct tctggacagc ctctagatcc tcgatgtaca tggaatcgga
      661 gttggcgagg gccagggcct tgagcgtcac aaactcctcc ttctccacct tgagcttctt
      721 gtacctgcgt accagctgca ggatggcccg gtagagctcc agcagccccg cgaggcggga
      781 gtgctcctca tccatgatgt agtcctcagc gtacaccagc ttgtc
//
