LOCUS       sequence-145936-        1035 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expresses LIC1 in mammalian cells; aa 389-523, untagged but cells 
            indicate expression with diffuse mCherry.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1035)
  AUTHORS   Schroeder CM, Ostrem JM, Hertz NT, Vale RD
  TITLE     A Ras-like domain in the light intermediate chain bridges the dynein
            motor to a cargo-binding region.
  JOURNAL   Elife. 2014 Oct 1;3:e03351. doi: 10.7554/eLife.03351.
  PUBMED    25272277
REFERENCE   2  (bases 1 to 1035)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2014-10-1"; volume: "3:e03351. doi"; pages: " 
            10.7554/eLife.03351"
COMMENT     Sequence Label: pHR-mCherry-p2A-LIC1 C-terminal half
FEATURES             Location/Qualifiers
     source          1..1035
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(107..133)
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             complement(551..607)
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     primer_bind     complement(898..917)
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtcaatcttt cacaaatttt gtaatccaga ggttgattat cgataagctt gatatcaagc
       61 ttgcatgcct gcaggtcgac tctagagtcg cggccgccat atgtcaagcg tagtctggga
      121 cgtcgtatgg gtaagaagct tctccttccg taggagatgt aggtgttgtg ggagaaactg
      181 taactggttt tcgtgtaatt ctgtctagtt ctgcatgaac atctaagaca ggcttctggc
      241 ctgacttttt ggtggatggt ggtaaaccac tgcttccacc tccagcccca cctgcagggc
      301 taccaccact cacaccaggg cctcctggag agccagtctt tttactcaac aaactgttga
      361 agaaatttgc cagaacgcct tcacttgtag ctccagcttt catgtttgga tcaatttttt
      421 ttgacccagc aggaatgggt gacacgctgg caacattaga tgatacagat ctatttggtg
      481 ttcgtgggga gcctcctggg actcttggtg aggcatccac aggccttcca gctgcagtca
      541 tttcggaccg aggtccaggg ttctcctcca cgtctccagc ctgcttcagc aggctgaagt
      601 tagtagctcc gcttccggat cccttgtaca gctcgtccat gccgccggtg gagtggcggc
      661 cctcggcgcg ttcgtactgt tccacgatgg tgtagtcctc gttgtgggag gtgatgtcca
      721 acttgatgtt gacgttgtag gcgccgggca gctgcacggg cttcttggcc ttgtaggtgg
      781 tcttgacctc agcgtcgtag tggccgccgt ccttcagctt cagcctctgc ttgatctcgc
      841 ccttcagggc gccgtcctcg gggtacatcc gctcggagga ggcctcccag cccatggtct
      901 tcttctgcat tacggggccg tcggagggga agttggtgcc gcgcagcttc accttgtaga
      961 tgaactcgcc gtcctgcagg gaggagtcct gggtcacggt caccacgccg ccgtcctcga
     1021 agttcatcac gcgct
//
