LOCUS       sequence-146007-       16531 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16531)
  AUTHORS   Cook, Jean
  TITLE     Huwe1 plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 16531)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pENTR1A-Huwe1 C4341S
FEATURES             Location/Qualifiers
     source          1..16531
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     terminator      103..189
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      281..308
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     primer_bind     316..339
                     /label=pENTR-F
                     /note="pENTR vectors, forward primer"
     protein_bind    358..457
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(14760..14859)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(14971..14990)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             14982..15791
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVSLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(15049..15068)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      15881..16469
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16370..16389
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgctagca tggatctcgg
       61 ggacgtctaa ctactaagcg agagtaggga actgccaggc atcaaataaa acgaaaggct
      121 cagtcggaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctcctgagt
      181 aggacaaatc cgccgggagc ggatttgaac gttgtgaagc aacggcccgg agggtggcgg
      241 gcaggacgcc cgccataaac tgccaggcat caaactaagc agaaggccat cctgacggat
      301 ggcctttttg cgtttctaca aactcttcct gttagttagt tacttaagct cgggccccaa
      361 ataatgattt tattttgact gatagtgacc tgttcgttgc aacaaattga taagcaatgc
      421 ttttttataa tgccaacttt gtacaaaaaa gcaggcttta aaggaaccaa ttcagtcgac
      481 atgaaagtag acaggactaa actgaagaag acacctactg aggctcctgc agactgcaga
      541 gccttaatag acaaactcaa agtttgtaat gatgagcaac ttctcttgga actgcagcag
      601 atcaaaacat ggaacattgg aaagtgcgag ttatatcact gggtggacct gttggaccgc
      661 ttcgatggaa tactggcaga tgctggacag acagtggaga atatgtcatg gatgctcgta
      721 tgtgataggc cagaaagaga gcaactgaaa atgcttctct tggctgtgtt gaacttcaca
      781 gccttgctca ttgagtacag cttttcccgg catctgtaca gttccataga gcatttgaca
      841 actttattgg cttcctctga tatgcaagtg gtgctggcag tcctcaatct cctatatgta
      901 tttagcaaaa gatcaaacta catcactcgt ctgggatctg acaagaggac cccgctgcta
      961 actcggctac aacatttggc agagagctgg ggtggaaagg agaatggctt tggacttgca
     1021 gaatgttgca gagacttgca tatgatgaaa tatccaccca gtgcaactac actacacttt
     1081 gaattctatg cagatcctgg ggccgaggtc aaaattgaga aaaggacaac tagtaacaca
     1141 ctacattata ttcacataga gcaacttgac aagatttcag aaagcccttc tgaaatcatg
     1201 gaatctctta ccaaaatgta cagcattcct aaggataagc agatgctgtt atttacacac
     1261 atacgactgg cccatggctt ttctaatcac aggaagcgat tgcaggcagt tcaggccaga
     1321 ctgcatgcaa tatctatatt agtgtattcc aatgccttgc aggaatcagc aaacagtatc
     1381 ttgtataatg gcttgataga ggagttggta gatgtccttc agataacgga taagcagctt
     1441 atggagatta aagcagcttc tttacgaaca ttaacatcaa ttgtccactt ggagagaact
     1501 cccaaactca gcagtattat tgactgtact ggaactgcct cctaccatgg atttttgcca
     1561 gtgcttgtaa ggaactgtat ccaggccatg attgatcctt ccatggatcc ataccctcac
     1621 cagtttgcca ctgctctctt ctctttttta taccatctgg ccagctacga tgctggtggt
     1681 gaagccttgg tctcctgtgg aatgatggaa gccttattga aggtcataaa gtttcttggc
     1741 gatgaacagg accagataac atttgtcacc agagccgtca gagtggttga ccttatcacc
     1801 aacctggata tggcagcttt tcaatcccat agtggacttt ctatcttcat ttatagactt
     1861 gagcatgaag tagatctgtg ccgaaaagaa tgtccgtttg tgatcaagcc aaagatccag
     1921 agacccaata ctacacaaga aggagaggaa atggaaactg atatggatgg agtccagtgt
     1981 attccacaac gagcagcact tctgaaatcc atgttgaatt tcctcaagaa ggccatccaa
     2041 gaccctgctt tctcagatgg catacgacat gtgatggatg gttctctgcc tacctccctg
     2101 aaacacatca tcagcaatgc agaatactat ggcccatcac tcttcctcct agctactgaa
     2161 gtggtgactg tgtttgtatt tcaagaacca tcactgctct cctcactcca ggacaatgga
     2221 ttgacagatg tcatgctgca tgcactgctt atcaaagatg ttcctgctac ccgtgaagtc
     2281 cttggctccc tcccaaatgt attcagtgca ctctgtttga atgcccgagg tcttcagtct
     2341 tttgttcagt gtcagccttt tgaacgcctc ttcaaagttc ttctgtctcc agattacctc
     2401 ccagccatgc ggaggaggag aagttctgat ccccttgggg atactgcatc caacctgggg
     2461 agtgctgtcg atgagctcat gagacatcag cccaccctta aaacagatgc aacgactgcc
     2521 atcatcaagt tacttgaaga aatctgtaat cttggaaggg accccaaata catctgtcag
     2581 aagccatcaa tccagaaggc agatggcact gccactgctc ctcccccaag gtctaatcat
     2641 gccgcagaag aagcctctag tgaggatgag gaggaagagg aagtacaggc catgcagagc
     2701 tttaattcta cccagcaaaa tgaaactgag cctaatcagc aggttgttgg tacagaggaa
     2761 cgtattccta ttcccctcat ggattacatc cttaatgtga tgaaatttgt ggaatctatt
     2821 ctgagcaaca atacaacaga tgaccactgc caggaatttg tgaatcagaa aggactgttg
     2881 cctttggtta ccattttggg tcttcccaat ctgcccattg actttcccac atctgctgcc
     2941 tgtcaggctg ttgcaggtgt ctgcaaatcc atattgacac tgtcacatga acccaaagtc
     3001 cttcaagagg gtctccttca gttggactcc atcctctcct ccctggagcc cttacaccgc
     3061 cccattgaat cccctggggg ctcagtgttg ttgcgagaac tggcttgcgc aggcaatgtt
     3121 gctgatgcta ccctctcagc ccaggccaca cctctgctgc atgcactcac cgcggcccat
     3181 gcctacatca tgatgtttgt tcatacttgc agagttggac agagtgaaat tcgttccatc
     3241 tccgtaaacc agtggggctc tcaattgggt ctgagtgttt tgagcaagct gagccagtta
     3301 tactgttccc tggtgtggga aagcactgtc ctcctctctc tgtgtacccc aaacagccta
     3361 ccatctgggt gtgaatttgg ccaggcagat atgcagaaac tggttccaaa ggatgagaag
     3421 gcaggtacga cccagggcgg aaaaagatca gatggggaac aggatggagc agctggaagt
     3481 atggatgctt ctacccaggg cttattagaa ggcattgggc tagatggtga cacattggct
     3541 cccatggaga cagatgaacc tactgcttca gactctaagg gcaaatctaa aatcacacca
     3601 gcaatggctg ccagaattaa gcaaatcaag cctttgttat cagcttcctc cagattaggc
     3661 cgagcacttg ctgagctatt tggacttctt gttaaacttt gtgtgggatc tcctgtccgc
     3721 cagagaagga gccatcatgc tgccagcacc actacagcac cgacacctgc cgcgcgatca
     3781 acagcctcag ctctcactaa gctcttgact aaggggttat cttggcagcc cccaccatat
     3841 acacctactc cccgattcag gctgacattc ttcatctgtt cagttggttt cacatcccca
     3901 atgctgtttg atgagaggaa gtatccctac cacctcatgc tgcaaaaatt tctctgctcc
     3961 ggaggccaca atgctctttt tgaaactttc aactgggctc tgtccatggg aggtaaagtt
     4021 cctgtttctg agggattgga acactcagac ttgcctgatg gcacaggaga attcctagat
     4081 gcctggctta tgctggtgga gaagatggtg aatcccacca cggtgcttga atctccacat
     4141 tcgctgcctg ccaaattgcc tggaggtgtc cagaactttc cccagttcag tgcactgcgc
     4201 ttccttgtgg taactcagaa agcagccttt acttgcatca aaaacttatg gaaccggaaa
     4261 cccctgaagg tatatggtgg acgaatggct gaatcgatgc tggccattct atgccacatc
     4321 ctccgaggag aacctgtgat tcgagagaga ctaagcaagg agaaggaggg gtctcgagga
     4381 gaagaggata cagggcaaga ggaaggtggc tcccgccggg aacctcaagt caaccagcaa
     4441 caactgcaac agctcatgga catgggcttc acaagggaac atgcaatgga ggcactgttg
     4501 aacaccagca ccatggagca ggccacagag taccttttaa cccaccctcc tccaatcatg
     4561 ggaggagttg ttcgggatct cagcatgtct gaagaggacc agatgatgag agcaattgct
     4621 atgtctctgg gacaggatat tccaatggat caaagggcag agtcacctga ggaagttgct
     4681 tgccggaagg aggaagagga acggaaagct cgggaaaagc aggaggagga agaggctaaa
     4741 tgtctagaga agttccagga tgctgacccg ttggaacaag atgagctcca cactttcaca
     4801 gatactatgt tgccaggctg cttccacctt cttgatgagc tgccagacac agtataccgt
     4861 gtgtgtgacc tgatcatgac agcaatcaaa cgtaatggag cagattatcg tgacatgatt
     4921 ctgaagcaag tagtcaatca ggtgtgggaa gctgctgatg tattgatcaa agctgctctt
     4981 cccctgacaa caagtgacac aaaaaccgtg tcagagtgga taagtcagat ggccacactg
     5041 ccccaggcct ccaatttggc tactagaatc ttgcttttaa cgctactttt tgaggagttg
     5101 aagctacctt gtgcttgggt ggttgaatca agtggcatcc ttaatgtcct aatcaaactc
     5161 ttggaagtgg ttcagccctg cctccaggca gccaaggagc agaaggaagt ccagacccca
     5221 aagtggatca caccagtgtt gctcctgatt gatttctatg aaaagacagc catctcctca
     5281 aaaaggagag cccagatgac taagtacctg caatccaaca gcaacaactg gcgctggttt
     5341 gatgatcgct ctgggcgttg gtgtagttac agtgcaagca acaatagcac tattgattct
     5401 gcctggaaat ctggagagac aagcgtgcga ttcactgcag gccgaagaag atacacggtc
     5461 caattcacta caatggtgca ggttaatgag gaaacaggga accgacgccc tgtgatgctg
     5521 actctcctca gggtacctcg gctgaataaa aattcaaaaa acagcaatgg acaggaacta
     5581 gagaagacgc tggaagaaag caaagaaatg gatatcaaac gtaaagaaaa taaaggcaat
     5641 gatacccctt tggccctaga gagtacaaac actgaaaagg agacaagcct ggaggaaaca
     5701 aaaatcgggg agatcctgat ccagggcttg acagaagata tggtgactgt tttaatccgg
     5761 gcctgcgtga gcatgctggg agtccctgtg gacccagata ctttgcatgc cacccttcgt
     5821 ctctgtctga ggctcacccg ggaccacaaa tatgccatga tgtttgcaga actgaagagt
     5881 acccgcatga tcttgaattt gacccagagc tcaggcttca atgggtttac tcccctggtc
     5941 acccttctct taagacacat cattgaggac ccctgtaccc ttcgtcatac catggaaaag
     6001 gttgttcgct cagcagctac aagtggagct ggtagcacta cctctggtgt tgtgtctggc
     6061 agcctcggct ctcgggagat caactacatc cttcgtgtcc ttgggccagc cgcatgccgc
     6121 aatccagaca tattcacaga agtggccaac tgctgtatcc gcatcgccct tcctgcccct
     6181 cgaggctcag gaactgcttc agatgatgaa tttgagaatc ttagaattaa aggccctaat
     6241 gctgtacagc tggtgaagac cacccctttg aagccctcac ctctgcctgt catccctgat
     6301 actatcaagg aagtgatcta tgatatgctg aatgctctgg ctgcatacca tgctccagag
     6361 gaagataaat ctgatcctaa acctggggtt atgacccaag aggttggcca gctcctgcaa
     6421 gacatgggtg atgatgtata ccagcagtac cggtcactta cgcgtcagag cagtgacttt
     6481 gatacgcagt caggtttttc cattaatagt caggtctttg ctgcagatgg tgcctccact
     6541 gagacttccg catctgggac ctcccaagga gaggcttcaa ctccagagga gtctcgagat
     6601 gggaagaaag ataaagaagg ggaccgggcc tctgaggaag gcaaacagaa aggcaagggc
     6661 agcaaacctt taatgcctac ctccactatc cttcgtcttc tggcagagtt ggtgaggtcc
     6721 tatgttggta ttgctaccct gattgccaac tacagctaca ctgtgggcca gtctgaactg
     6781 atcaaagagg actgcagtgt gctagctttt gttctggacc acctgctccc acatacccag
     6841 aatgcagaag acaaggacac ccctgccttg gcccgcctgt tcctcgcaag cctggctgct
     6901 gcagggagtg gcacagatgc ccaggtggcc ctagtgaatg aagtaaaagc agcccttgga
     6961 cgggcactgg ctatggctga gagtacagag aaacatgcca ggcttcaggc agtgatgtgt
     7021 atcatcagta ctatcatgga gtcctgcccc tccacctcca gcttctacag cagtgccaca
     7081 gcgaagaccc agcacaatgg catgaacaac atcattcggc ttttcctgaa gaagggactg
     7141 gttaatgacc tggccagagt acctcacagc ttagacctgt ccagtcccaa catggccaac
     7201 acagtcaatg ctgctctgaa gcctttggaa acactttccc ggattgtgaa ccagcccagt
     7261 agcctttttg gcagcaagag tgcttctagc aagaacaagt ctgagcagga tgcccaagga
     7321 gcctctcaag attccagtag caaccagcag gacccaggcg agcctgggga agcagaagtg
     7381 caggaggagg atcatgatgt cactcacaca gaggtggcag atggggatat catggatggg
     7441 gaggctgaaa ccgactcagt ggtgattgct gggcagcctg aggtgctcag ttcacaagag
     7501 atgcaggttg agaatgagct ggaggacctg atagatgagt tgcttgagag ggatggcgga
     7561 tctgggaaca gtacaattat agtgagcaga agtggagagg atgaatcaca agaggacgtg
     7621 ctgatggatg aagctccttc caacctcagc caagcttcca ccttgcaggc caaccgagaa
     7681 gattccatga atatcctgga ccctgaggat gaggaggagc acactcagga agaggacagc
     7741 agtggcagta acgaggatga ggatgatagt caggatgaag aggaggagga ggaggaagat
     7801 gaggaagatg atcaggagga tgatgaaggt gaagagggag atgaagacga tgacgacgat
     7861 ggctctgaga tggaattgga tgaggattat cctgatatga acgcttctcc cttggtccga
     7921 tttgagcgct ttgaccggga ggatgatctc atcattgagt ttgacaacat gttctccagt
     7981 gctacagaca tccccccatc cccaggaaat atccctacca cccatccact gatggtgcgc
     8041 catgcagacc acagttctct gacactgggc agtggctctt caacaactcg tctcacccag
     8101 ggcatcgggc gcagtcagag gaccctaagg cagctgacgg ccaatactgg ccacaccatt
     8161 catgttcact accctgggaa tcgccagccc aaccctcctc ttatactgca gaggttgctt
     8221 ggtccctcag ctgctgctga catccttcag ctgagcagca gccttcccct acaaagccgg
     8281 ggtcgggccc gcctcctggt aggcaacgat gacgtccaca tcatcgcccg ttctgatgat
     8341 gagctgctgg atgacttttt ccatgatcag agcacagcta ccagccaagc aggaaccctg
     8401 tccagcatcc ccacagccct gacccgctgg acagaagaat gcaaagttct cgatgctgag
     8461 agcatgcatg actgtgtttc agtggttaaa gtgtccattg tcaatcacct ggaattcctg
     8521 agggatgagg agctggaaga aaggcgagag aagcgcagga aacaactggc tgaggaagaa
     8581 acaaagataa ctgataaagg caaagaagat aaggagaaca gggatcagag tgcacagtgt
     8641 actgcatcta agtcaaatga ctccactgaa cagaatctct cagatgggac gcctatgcct
     8701 gacagctacc caacaacccc atcttcaact gatgcagcta catctgagtc caaggagacc
     8761 cttggcactc tgcaatcctc acaacagcaa ccaacactcc caaccccacc agctttggga
     8821 gaggttcctc aggagctgca gtctccagct ggagaagggg gcagctctac acagctattg
     8881 atgcctgtag agccagagga attgggtccc acaaggccaa gtggggaagc agaaacaact
     8941 cagatggagt tatccccagc tcccactata acctcacttt ccccagagag agctgaggat
     9001 tctgatgcac tgacggctgt cagcagtcag ctagaaggct ctcctatgga tacaagcagc
     9061 ctggcttcct gtaccttaga ggaggctgtg ggtgacactt cagcagctgg cagttctgag
     9121 cagcccagag caggcagctc cactcctggg gatgccccac cagctgtggc ggaagtgcaa
     9181 ggcaggagtg atgggtcagg ggaatctgcc cagccacctg aggacagctc cccacctgca
     9241 tcctctgaga gctcttccac cagagattct gccgtggcca tttctggagc agattcccga
     9301 ggaatcctag aagagccgtt gccttcaaca agcagtgaag aagaagatcc ccttgcgggt
     9361 atcagtctcc ctgaaggtgt ggacccctct tttctggctg ccctgcctga tgacatccgt
     9421 cgggaagttc tacagaacca gctaggcatt cgtccaccaa cccggactgc cccctccaca
     9481 aatagctcag cgcctgcagt ggtggggaat cctggtgtga ctgaagtact ggcacagcag
     9541 agagctgagc agcagcgacg agaactagca cagaatgcca gctcagacac ccctatggac
     9601 cctgtgacct tcatccagac tctgccctca gacctgcgcc gtagtgtcct agaggatatg
     9661 gaggacagtg tgttagctgt gatgccacct gacattgcag ctgaggctca agccctgaga
     9721 cgagagcaag aagcccggca gcgacagctc atgcatgagc gtctgtttgg gcacagtagc
     9781 acctccgcac tctctgctat tctccgaagc ccggctttca ccagtcgctt aagtggcaac
     9841 cgtggggtcc agtatactcg ccttgctgtg cagagaggtg gcaccttcca gatggggggt
     9901 agcagcagcc ataacaggcc ttctggcagt aatgtagata ctctcctccg cctccgagga
     9961 cggctccttc tggaccacga agccctttct tgtctcttgg tcctactttt tgtggatgag
    10021 ccaaagctca atactagccg tctacaccga gtactgagaa atctctgcta ccatgcccag
    10081 acccgccact gggtcatccg cagtctgctc tccatcttgc agcgcagcag tgagagtgag
    10141 ctatgcattg aaacacccaa actcactaca agtgaggaaa agggcaaaaa gtcgagcaag
    10201 agctgtgggt caagtagcca tgagaaccgt cccctggacc tgctacacaa gatggagtca
    10261 aagagctcca accagctttc ctggctctca gtatccatgg atgcagccct aggctgcagg
    10321 actaatatat ttcagatcca gcgttcaggg gggcgtaaac ataccgagaa gcatgcaagc
    10381 ggtggctcca ccgtccacat ccatccccaa gctgctcctg ttgtctgcag acacgttttg
    10441 gatacactca ttcaattggc caaggtattt cccagccact tcacacagca gcggaccaaa
    10501 gaaacaaact gtgagagtga tcgggaaagg ggcaataagg cctgtagccc atgctcctca
    10561 cagtcctcca gcagtggcat ttgcacagac ttctgggact tattggtaaa actggacaac
    10621 atgaatgtca gccggaaagg caagaactcc gtgaagtcag tgccagtgag cgctggcggt
    10681 gagggggaaa cctctccata cagcctcgag gcctctccac tggggcagct catgaacatg
    10741 ttgtcacacc cagtcatccg ccggagctct ctcttaactg agaaactcct cagactcctt
    10801 tctctcatct caattgctct cccagaaaac aaggtgtcag aagcacaggc taattctggc
    10861 agcggtgctt cctccaccac cactgccacc tcaaccacat ctaccaccac caccactgcc
    10921 gcctccacca cgcccacacc ccctactgca cccacccctg tcacttctgc tccagccctg
    10981 gttgctgcca cggctatttc caccattgtc gtagctgctt cgaccacagt gactaccccc
    11041 acgactgcta ccactactgt ttcaatttct cccactacta agggcagcaa atctccagcg
    11101 aaggtgagtg atgggggcag cagcagtaca gactttaaga tggtgtcctc tggcctcact
    11161 gaaaaccagc tacagctctc tgtagaggtg ttgacatccc actcttgttc tgaggaaggc
    11221 ttagaggatg cagccaacgt actactgcag ctctcccggg gggactctgg gacccgggac
    11281 actgttctca agctgctact gaatggagcc cgccatctgg gttataccct ttgtaaacaa
    11341 ataggtaccc tgctggccga gctgcgggaa tacaacctcg agcagcagcg gcgagcccaa
    11401 tgtgaaaccc tctctcctga tggcctgcct gaggagcagc cacagaccac caagctgaag
    11461 ggcaaaatgc agagcaggtt tgacatggct gagaatgtgg taattgtggc atctcagaag
    11521 cgacctttgg gtggccggga gctccagctg ccttctatgt ccatgttgac atccaagaca
    11581 tctacccaga agttcttctt gagggtacta caggtcatca tccagctccg ggacgacacg
    11641 cgccgggcta acaagaaagc caagcagaca ggcaggctag gttcctccgg tttaggctca
    11701 gctagcagca tccaggcagc tgttcggcag ctggaggctg aggctgatgc cattatacaa
    11761 atggtacgtg agggtcaaag ggcgcggaga cagcaacaag cagcaacgtc ggagtctagc
    11821 cagtcagagg cgtctgtccg gagggaggaa tcacccatgg atgtggacca gccatctccc
    11881 agtgctcaag atactcaatc cattgcctcc gatggaaccc cacaggggga gaaggaaaag
    11941 gaagaaagac cacctgagtt acccctgctc agcgagcagc tgagtttgga cgagctgtgg
    12001 gacatgcttg gggagtgtct aaaggaacta gaggaatccc atgaccagca tgcggtgcta
    12061 gtgctacagc ctgctgtcga ggccttcttt ctggtccatg ccacagagcg ggagagcaag
    12121 cctcctgtcc gagacacccg tgagagccag ctggcacaca tcaaggacga gcctcctcca
    12181 ctctcccctg cccccttaac cccagccacg ccttcctccc ttgacccatt cttctcccgg
    12241 gagccctcat ctatgcacat ctcctcaagc ctgccccctg acacacagaa gttccttcgc
    12301 tttgcagaga ctcaccgcac tgtgttaaac cagatcctac ggcagtccac gacccacctt
    12361 gctgatgggc cttttgctgt cctggtagac tacattcgtg tcctcgactt tgatgtcaag
    12421 cgcaaatatt tccgccaaga gctggagcgt ttagatgagg ggctccggaa agaagacatg
    12481 gctgtgcatg tccgtcgtga ccatgtgttt gaagactcct atcgtgagct gcatcgcaaa
    12541 tcccccgaag aaatgaagaa tcgattgtat atagtatttg aaggagaaga agggcaggat
    12601 gctggcgggc tcctgcggga gtggtatatg atcatctctc gagagatgtt taaccctatg
    12661 tatgccttgt tccgtacctc acctggtgat cgagtcacct acaccatcaa tccatcttcc
    12721 cactgcaacc ccaaccacct cagctacttc aagtttgtcg gacgcattgt ggccaaagct
    12781 gtatatgaca accgtcttct ggagtgctac tttactcgat ccttttacaa acacatcttg
    12841 ggcaagtcag tcagatatac agatatggag agtgaagatt accacttcta ccaaggtctg
    12901 gtttatctgc tggaaaatga tgtctccaca ctaggctatg acctcacctt cagcactgag
    12961 gtccaagagt ttggagtttg tgaagttcgt gacctcaaac ccaatggggc caacatcttg
    13021 gtaacagagg agaataagaa ggagtatgta cacctggtat gccagatgag aatgacagga
    13081 gccatccgca agcagttggc ggctttctta gaaggcttct atgagatcat tccaaagcgc
    13141 ctcatttcca tcttcactga gcaggagtta gagctgctta tatcaggact gcccaccatt
    13201 gacatcgatg atctgaaatc caacactgaa taccacaagt accagtccaa ctctattcag
    13261 atccagtggt tctggagagc attgcgttct ttcgatcaag ctgaccgtgc caagttcctc
    13321 cagtttgtca cgggtacttc caaggtaccc ctgcaaggct ttgctgccct cgaaggcatg
    13381 aatggcattc agaagtttca gatccatcga gatgacaggt ccacagatcg cctgccttca
    13441 gctcacacaa gctttaatca gctggatctg cctgcctatg agagctttga gaagctccgc
    13501 cacatgctac tgttggctat ccaggagtgc tctgaaggct ttgggctggc ctaataaggc
    13561 cctgcccaac tccgtggggt tttttttacc attgttggac ctggggaggg gggagttaaa
    13621 aaaagaacca gaaagaaatt gtcaaaaacc aataaatgaa atccaccaac tcaccgtgtg
    13681 tgtcccagct gccccatctt ccccagcgca tacctgttcc tcttctcatt ctctccccgc
    13741 cgcctgtttc ctcaccttct ctcccctttc catgccgtcc atgatcccca ccccatgtgt
    13801 tttaaaaagg cagtagcctt tgcagggacc tgtctgtccc aactgtttga acagtgtgct
    13861 cctcagattc tgtgttcaga aggatttgct gcattgagac ttgaaacctt tggatagggg
    13921 aaaaaattat atatatatat atttttttgt tctgtttgca tttcttaatt tgtgcttgga
    13981 atgtgttgat gtgcacagct aatgattcaa tgcgagacaa gattggcgtc tgtgttgtgg
    14041 aggtttcaaa taaagagcac tcttcataaa aaaaaaaaaa aaaacaaaaa actcttaagc
    14101 acatgctagg catctggaga aaagatggat tctccaggaa accatccctg ccctacccgc
    14161 ttgcccacct gcctccacca ctggtgcaac ttcctccttc agagtcagtt tctgcagtca
    14221 ggagagatga gcagttcacc aggaattggg ggtgggggca tctgtttttt ttttttagaa
    14281 tgggagctgc acttggggat ggtgatggtg ttagagattg cagcccagga caaagcatca
    14341 ccttgctcaa ggggaacaaa ctgcagcagg ttgtacaaaa cattcagaga gctcacttga
    14401 cccaaccgag ggttttactt ggtgagcctt tctagtagtc ttgagtctgg ggctcagttt
    14461 tagattttta tttcattaat gtttgtttat ttctaataaa ttttaataag cgagtaattt
    14521 aactaggtcg ccagaagacc attatttttg tttgtctatt tttgttgtcg tcactccctc
    14581 tctcgtcatc tgtctgccag tctgttcaca gatcaccttg tagtcccttg tggtttttac
    14641 tccatccagt gcttaaagct gatctaagga cttcgatggt tccagccttc aaagaaaaaa
    14701 taatttaata aaaatttagt tagaaaaaat aaccccgcgg ccgcactcga gatatctaga
    14761 cccagctttc ttgtacaaag ttggcattat aagaaagcat tgcttatcaa tttgttgcaa
    14821 cgaacaggtc actatcagtc aaaataaaat cattatttgc catccagctg cagctctggc
    14881 ccgtgtctca aaatctctga tgttacattg cacaagataa aaatatatca tcatgaacaa
    14941 taaaactgtc tgcttacata aacagtaata caaggggtgt tatgagccat attcaacggg
    15001 aaacgtcgag gccgcgatta aattccaaca tggatgctga tttatatggg tataaatggg
    15061 ctcgcgataa tgtcgggcaa tcaggtgcga caatctatcg cttgtatggg aagcccgatg
    15121 cgccagagtt gtttctgaaa catggcaaag gtagcgttgc caatgatgtt acagatgaga
    15181 tggtcagact aaactggctg acggaattta tgcctcttcc gaccatcaag cattttatcc
    15241 gtactcctga tgatgcatgg ttactcacca ctgcgatccc cggaaaaaca gcattccagg
    15301 tattagaaga atatcctgat tcaggtgaaa atattgttga tgcgctggca gtgtccctgc
    15361 gccggttgca ttcgattcct gtttgtaatt gtccttttaa cagcgatcgc gtatttcgtc
    15421 tcgctcaggc gcaatcacga atgaataacg gtttggttga tgcgagtgat tttgatgacg
    15481 agcgtaatgg ctggcctgtt gaacaagtct ggaaagaaat gcataaactt ttgccattct
    15541 caccggattc agtcgtcact catggtgatt tctcacttga taaccttatt tttgacgagg
    15601 ggaaattaat aggttgtatt gatgttggac gagtcggaat cgcagaccga taccaggatc
    15661 ttgccatcct atggaactgc ctcggtgagt tttctccttc attacagaaa cggctttttc
    15721 aaaaatatgg tattgataat cctgatatga ataaattgca gtttcatttg atgctcgatg
    15781 agtttttcta atcagaattg gttaattggt tgtaacatta ttcagattgg gccccgttcc
    15841 actgagcgtc agacccggta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
    15901 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
    15961 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
    16021 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
    16081 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
    16141 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
    16201 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
    16261 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
    16321 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
    16381 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
    16441 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
    16501 tggccttttg ctggcctttt gctcacatgt t
//
