LOCUS       sequence-146075-         798 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PiggyBac expression gateway destination vector with 
            Insulator-flanked EF1a promoter gateway cassette and floxed Hygro 
            selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 798)
  AUTHORS   Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan 
            S, Shivalila CS, Dadon DB, Jaenisch R
  TITLE     Multiplexed activation of endogenous genes by CRISPR-on, an 
            RNA-guided transcriptional activator system.
  JOURNAL   Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
  PUBMED    23979020
REFERENCE   2  (bases 1 to 798)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Res. 
            2013 Aug 27. doi: 10.1038/cr.2013.122."
COMMENT     Sequence Label: pAC150-PBLHL-4xHS-EF1a-DEST
FEATURES             Location/Qualifiers
     source          1..798
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    58..182
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(207..212,213..230,231..237)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
     primer_bind     complement(357..376)
                     /label=CAT-R
                     /note="Chloramphenicol resistance gene, reverse primer"
ORIGIN
        1 agcttggtac cgagctcgga tccactagtc cagtgtggtg gaattctgca gatatcaaca
       61 agtttgtaca aaaaagctga acgagaaacg taaaatgata taaatatcaa tatattaaat
      121 tagattttgc ataaaaaaca gactacataa tactgtaaaa cacaacatat ccagtcacta
      181 tggcggccgc attaggcacc ccaggcttta cactttatgc ttccggctcg tataatgtgt
      241 ggattttgag ttaggatccg tcgagatttt caggagctaa ggaagctaaa atggagaaaa
      301 aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa cattttgagg
      361 catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat attacggcct
      421 ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt cacattcttg
      481 cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt gagctggtga
      541 tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa acgttttcat
      601 cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat tcgcaagatg
      661 tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag aatatgtttt
      721 tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg gccaatatgg
      781 acaacttctt cgcccccg
//
