LOCUS       sequence-146187-         901 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses  CrTRP1 in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 901)
  AUTHORS   Arias-Darraz L, Cabezas D, Colenso CK, Alegria-Arcos M, Bravo-Moraga
            F, Varas-Concha I, Almonacid DE, Madrid R, Brauchi S
  TITLE     A transient receptor potential ion channel in Chlamydomonas shares 
            key features with sensory transduction-associated TRP channels in 
            mammals.
  JOURNAL   Plant Cell. 2015 Jan;27(1):177-88. doi: 10.1105/tpc.114.131862. Epub
            2015 Jan 16.
  PUBMED    25595824
REFERENCE   2  (bases 1 to 901)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1105/tpc.114.131862"; journalName: "Plant Cell"; date: 
            "2015-01"; volume: "27"; issue: "1"; pages: "177-88"
COMMENT     Sequence Label: pTracer_Cr-TRP1
FEATURES             Location/Qualifiers
     source          1..901
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             join(8..10,11..13,14..526)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(53..74)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(314..333)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     661..682
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tacttagatg gtgagcaagg gcgaggagct gttcaccggg gtggtgccca tcctggtcga
       61 gctggacggc gacgtaaacg gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc
      121 cacctacggc aagctgaccc tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg
      181 gcccaccctc gtgaccaccc tgacctacgg cgtgcagtgc ttcagccgct accccgacca
      241 catgaagcag cacgacttct tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac
      301 catcttcttc aaggacgacg gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga
      361 caccctggtg aaccgcatcg agctgaaggg catcgacttc aaggaggacg gcaacatcct
      421 ggggcacaag ctggagtaca actacaacag ccacaacgtc tatatcatgg ccgacaagca
      481 gaagaacggc atcaaggtga acttcaagat ccgccacaac atcgaggacg gcagcgtgca
      541 gctcgccgac cactaccagc agaacacccc catcggcgac ggccccgtgc tgctgcccga
      601 caaccactac ctgagcaccc agtccgccct gagcaaagac cccaacgaga agcgcgatca
      661 catggtcctg ctggagttcg tgacccgccg ccgggatcac tctcggcatg gacgagctgt
      721 acaagggcgc aggtgccggg gccaagcttg gtaccagcga catgaaggtc gctccagcgc
      781 cagcctcagg gcagccctgg cggaggcagc aagaagccat cggactatgg gtgccagctg
      841 cactacaagc acgcgccgcg tggnggaagc cggagtccac cacggatgaa ggcatgaagc
      901 g
//
